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Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
The sorting of transmembrane cargo proteins into the lumenal vesicles of multivesicular bodies (MVBs) depends on the recruitment of endosomal sorting complexes required for transport (ESCRTs) to the cytosolic face of endosomal membranes. The subsequent dissociation of ESCRT complexes from endosomes...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064671/ https://www.ncbi.nlm.nih.gov/pubmed/17130288 http://dx.doi.org/10.1083/jcb.200606113 |
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author | Nickerson, Daniel P. West, Matthew Odorizzi, Greg |
author_facet | Nickerson, Daniel P. West, Matthew Odorizzi, Greg |
author_sort | Nickerson, Daniel P. |
collection | PubMed |
description | The sorting of transmembrane cargo proteins into the lumenal vesicles of multivesicular bodies (MVBs) depends on the recruitment of endosomal sorting complexes required for transport (ESCRTs) to the cytosolic face of endosomal membranes. The subsequent dissociation of ESCRT complexes from endosomes requires Vps4, a member of the AAA family of adenosine triphosphatases. We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II. Surprisingly, vesicle budding into the endosome lumen occurs in the absence of Did2 function even though Did2 is required for the efficient sorting of MVB cargo proteins into lumenal vesicles. This uncoupling of MVB cargo sorting and lumenal vesicle formation suggests that the Vps4-mediated dissociation of ESCRT-III is an essential step in the sorting of cargo proteins into MVB vesicles but is not a prerequisite for the budding of vesicles into the endosome lumen. |
format | Text |
id | pubmed-2064671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20646712007-11-29 Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes Nickerson, Daniel P. West, Matthew Odorizzi, Greg J Cell Biol Research Articles The sorting of transmembrane cargo proteins into the lumenal vesicles of multivesicular bodies (MVBs) depends on the recruitment of endosomal sorting complexes required for transport (ESCRTs) to the cytosolic face of endosomal membranes. The subsequent dissociation of ESCRT complexes from endosomes requires Vps4, a member of the AAA family of adenosine triphosphatases. We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II. Surprisingly, vesicle budding into the endosome lumen occurs in the absence of Did2 function even though Did2 is required for the efficient sorting of MVB cargo proteins into lumenal vesicles. This uncoupling of MVB cargo sorting and lumenal vesicle formation suggests that the Vps4-mediated dissociation of ESCRT-III is an essential step in the sorting of cargo proteins into MVB vesicles but is not a prerequisite for the budding of vesicles into the endosome lumen. The Rockefeller University Press 2006-12-04 /pmc/articles/PMC2064671/ /pubmed/17130288 http://dx.doi.org/10.1083/jcb.200606113 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Nickerson, Daniel P. West, Matthew Odorizzi, Greg Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes |
title | Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes |
title_full | Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes |
title_fullStr | Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes |
title_full_unstemmed | Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes |
title_short | Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes |
title_sort | did2 coordinates vps4-mediated dissociation of escrt-iii from endosomes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064671/ https://www.ncbi.nlm.nih.gov/pubmed/17130288 http://dx.doi.org/10.1083/jcb.200606113 |
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