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Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes

The sorting of transmembrane cargo proteins into the lumenal vesicles of multivesicular bodies (MVBs) depends on the recruitment of endosomal sorting complexes required for transport (ESCRTs) to the cytosolic face of endosomal membranes. The subsequent dissociation of ESCRT complexes from endosomes...

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Detalles Bibliográficos
Autores principales: Nickerson, Daniel P., West, Matthew, Odorizzi, Greg
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064671/
https://www.ncbi.nlm.nih.gov/pubmed/17130288
http://dx.doi.org/10.1083/jcb.200606113
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author Nickerson, Daniel P.
West, Matthew
Odorizzi, Greg
author_facet Nickerson, Daniel P.
West, Matthew
Odorizzi, Greg
author_sort Nickerson, Daniel P.
collection PubMed
description The sorting of transmembrane cargo proteins into the lumenal vesicles of multivesicular bodies (MVBs) depends on the recruitment of endosomal sorting complexes required for transport (ESCRTs) to the cytosolic face of endosomal membranes. The subsequent dissociation of ESCRT complexes from endosomes requires Vps4, a member of the AAA family of adenosine triphosphatases. We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II. Surprisingly, vesicle budding into the endosome lumen occurs in the absence of Did2 function even though Did2 is required for the efficient sorting of MVB cargo proteins into lumenal vesicles. This uncoupling of MVB cargo sorting and lumenal vesicle formation suggests that the Vps4-mediated dissociation of ESCRT-III is an essential step in the sorting of cargo proteins into MVB vesicles but is not a prerequisite for the budding of vesicles into the endosome lumen.
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spelling pubmed-20646712007-11-29 Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes Nickerson, Daniel P. West, Matthew Odorizzi, Greg J Cell Biol Research Articles The sorting of transmembrane cargo proteins into the lumenal vesicles of multivesicular bodies (MVBs) depends on the recruitment of endosomal sorting complexes required for transport (ESCRTs) to the cytosolic face of endosomal membranes. The subsequent dissociation of ESCRT complexes from endosomes requires Vps4, a member of the AAA family of adenosine triphosphatases. We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II. Surprisingly, vesicle budding into the endosome lumen occurs in the absence of Did2 function even though Did2 is required for the efficient sorting of MVB cargo proteins into lumenal vesicles. This uncoupling of MVB cargo sorting and lumenal vesicle formation suggests that the Vps4-mediated dissociation of ESCRT-III is an essential step in the sorting of cargo proteins into MVB vesicles but is not a prerequisite for the budding of vesicles into the endosome lumen. The Rockefeller University Press 2006-12-04 /pmc/articles/PMC2064671/ /pubmed/17130288 http://dx.doi.org/10.1083/jcb.200606113 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Nickerson, Daniel P.
West, Matthew
Odorizzi, Greg
Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
title Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
title_full Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
title_fullStr Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
title_full_unstemmed Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
title_short Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes
title_sort did2 coordinates vps4-mediated dissociation of escrt-iii from endosomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064671/
https://www.ncbi.nlm.nih.gov/pubmed/17130288
http://dx.doi.org/10.1083/jcb.200606113
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