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Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis

The budding yeast regulation of Ace2 and morphogenesis (RAM) network integrates cell fate determination and morphogenesis. Its disruption impairs polarized growth and causes mislocalization of the transcription factor Ace2, resulting in failure of daughter cell–specific transcription required for ce...

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Detalles Bibliográficos
Autores principales: Jansen, Jaclyn M., Barry, Margaret F., Yoo, Charles K., Weiss, Eric L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064675/
https://www.ncbi.nlm.nih.gov/pubmed/17145962
http://dx.doi.org/10.1083/jcb.200604107
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author Jansen, Jaclyn M.
Barry, Margaret F.
Yoo, Charles K.
Weiss, Eric L.
author_facet Jansen, Jaclyn M.
Barry, Margaret F.
Yoo, Charles K.
Weiss, Eric L.
author_sort Jansen, Jaclyn M.
collection PubMed
description The budding yeast regulation of Ace2 and morphogenesis (RAM) network integrates cell fate determination and morphogenesis. Its disruption impairs polarized growth and causes mislocalization of the transcription factor Ace2, resulting in failure of daughter cell–specific transcription required for cell separation. We find that phosphoregulation of the conserved AGC family kinase Cbk1 is critical for RAM network function. Intramolecular autophosphorylation of the enzyme's activation loop is critical for kinase activity but is only partially required for cell separation and polarized growth. In marked contrast, phosphorylation of a C-terminal hydrophobic motif is required for Cbk1 function in vivo but not for its kinase activity, suggesting a previously unappreciated level of control for this family of kinases. Phosphorylation of the C-terminal site is regulated over the cell cycle and requires the transcription factor Ace2 as well as all RAM network components. Therefore, Ace2 is not only a downstream target of Cbk1 but also reinforces activation of its upstream regulator.
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spelling pubmed-20646752007-11-29 Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis Jansen, Jaclyn M. Barry, Margaret F. Yoo, Charles K. Weiss, Eric L. J Cell Biol Research Articles The budding yeast regulation of Ace2 and morphogenesis (RAM) network integrates cell fate determination and morphogenesis. Its disruption impairs polarized growth and causes mislocalization of the transcription factor Ace2, resulting in failure of daughter cell–specific transcription required for cell separation. We find that phosphoregulation of the conserved AGC family kinase Cbk1 is critical for RAM network function. Intramolecular autophosphorylation of the enzyme's activation loop is critical for kinase activity but is only partially required for cell separation and polarized growth. In marked contrast, phosphorylation of a C-terminal hydrophobic motif is required for Cbk1 function in vivo but not for its kinase activity, suggesting a previously unappreciated level of control for this family of kinases. Phosphorylation of the C-terminal site is regulated over the cell cycle and requires the transcription factor Ace2 as well as all RAM network components. Therefore, Ace2 is not only a downstream target of Cbk1 but also reinforces activation of its upstream regulator. The Rockefeller University Press 2006-12-04 /pmc/articles/PMC2064675/ /pubmed/17145962 http://dx.doi.org/10.1083/jcb.200604107 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Jansen, Jaclyn M.
Barry, Margaret F.
Yoo, Charles K.
Weiss, Eric L.
Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis
title Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis
title_full Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis
title_fullStr Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis
title_full_unstemmed Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis
title_short Phosphoregulation of Cbk1 is critical for RAM network control of transcription and morphogenesis
title_sort phosphoregulation of cbk1 is critical for ram network control of transcription and morphogenesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064675/
https://www.ncbi.nlm.nih.gov/pubmed/17145962
http://dx.doi.org/10.1083/jcb.200604107
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