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Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast

Autophagy is a conserved degradative pathway that is induced in response to various stress and developmental conditions in eukaryotic cells. It allows the elimination of cytosolic proteins and organelles in the lysosome/vacuole. In the yeast Saccharomyces cerevisiae, the integral membrane protein At...

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Autores principales: He, Congcong, Song, Hui, Yorimitsu, Tomohiro, Monastyrska, Iryna, Yen, Wei-Lien, Legakis, Julie E., Klionsky, Daniel J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064702/
https://www.ncbi.nlm.nih.gov/pubmed/17178909
http://dx.doi.org/10.1083/jcb.200606084
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author He, Congcong
Song, Hui
Yorimitsu, Tomohiro
Monastyrska, Iryna
Yen, Wei-Lien
Legakis, Julie E.
Klionsky, Daniel J.
author_facet He, Congcong
Song, Hui
Yorimitsu, Tomohiro
Monastyrska, Iryna
Yen, Wei-Lien
Legakis, Julie E.
Klionsky, Daniel J.
author_sort He, Congcong
collection PubMed
description Autophagy is a conserved degradative pathway that is induced in response to various stress and developmental conditions in eukaryotic cells. It allows the elimination of cytosolic proteins and organelles in the lysosome/vacuole. In the yeast Saccharomyces cerevisiae, the integral membrane protein Atg9 (autophagy-related protein 9) cycles between mitochondria and the preautophagosomal structure (PAS), the nucleating site for formation of the sequestering vesicle, suggesting a role in supplying membrane for vesicle formation and/or expansion during autophagy. To better understand the mechanisms involved in Atg9 cycling, we performed a yeast two-hybrid–based screen and identified a peripheral membrane protein, Atg11, that interacts with Atg9. We show that Atg11 governs Atg9 cycling through the PAS during specific autophagy. We also demonstrate that the integrity of the actin cytoskeleton is essential for correct targeting of Atg11 to the PAS. We propose that a pool of Atg11 mediates the anterograde transport of Atg9 to the PAS that is dependent on the actin cytoskeleton during yeast vegetative growth.
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spelling pubmed-20647022007-11-29 Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast He, Congcong Song, Hui Yorimitsu, Tomohiro Monastyrska, Iryna Yen, Wei-Lien Legakis, Julie E. Klionsky, Daniel J. J Cell Biol Research Articles Autophagy is a conserved degradative pathway that is induced in response to various stress and developmental conditions in eukaryotic cells. It allows the elimination of cytosolic proteins and organelles in the lysosome/vacuole. In the yeast Saccharomyces cerevisiae, the integral membrane protein Atg9 (autophagy-related protein 9) cycles between mitochondria and the preautophagosomal structure (PAS), the nucleating site for formation of the sequestering vesicle, suggesting a role in supplying membrane for vesicle formation and/or expansion during autophagy. To better understand the mechanisms involved in Atg9 cycling, we performed a yeast two-hybrid–based screen and identified a peripheral membrane protein, Atg11, that interacts with Atg9. We show that Atg11 governs Atg9 cycling through the PAS during specific autophagy. We also demonstrate that the integrity of the actin cytoskeleton is essential for correct targeting of Atg11 to the PAS. We propose that a pool of Atg11 mediates the anterograde transport of Atg9 to the PAS that is dependent on the actin cytoskeleton during yeast vegetative growth. The Rockefeller University Press 2006-12-18 /pmc/articles/PMC2064702/ /pubmed/17178909 http://dx.doi.org/10.1083/jcb.200606084 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
He, Congcong
Song, Hui
Yorimitsu, Tomohiro
Monastyrska, Iryna
Yen, Wei-Lien
Legakis, Julie E.
Klionsky, Daniel J.
Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
title Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
title_full Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
title_fullStr Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
title_full_unstemmed Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
title_short Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
title_sort recruitment of atg9 to the preautophagosomal structure by atg11 is essential for selective autophagy in budding yeast
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064702/
https://www.ncbi.nlm.nih.gov/pubmed/17178909
http://dx.doi.org/10.1083/jcb.200606084
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