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JunB is required for endothelial cell morphogenesis by regulating core-binding factor β
The molecular mechanism triggering the organization of endothelial cells (ECs) in multicellular tubules is mechanistically still poorly understood. We demonstrate that cell-autonomous endothelial functions of the AP-1 subunit JunB are required for proper endothelial morphogenesis both in vivo in mou...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064707/ https://www.ncbi.nlm.nih.gov/pubmed/17158955 http://dx.doi.org/10.1083/jcb.200605149 |
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author | Licht, Alexander H. Pein, Oliver T. Florin, Lore Hartenstein, Bettina Reuter, Hendrik Arnold, Bernd Lichter, Peter Angel, Peter Schorpp-Kistner, Marina |
author_facet | Licht, Alexander H. Pein, Oliver T. Florin, Lore Hartenstein, Bettina Reuter, Hendrik Arnold, Bernd Lichter, Peter Angel, Peter Schorpp-Kistner, Marina |
author_sort | Licht, Alexander H. |
collection | PubMed |
description | The molecular mechanism triggering the organization of endothelial cells (ECs) in multicellular tubules is mechanistically still poorly understood. We demonstrate that cell-autonomous endothelial functions of the AP-1 subunit JunB are required for proper endothelial morphogenesis both in vivo in mouse embryos with endothelial-specific ablation of JunB and in in vitro angiogenesis models. By cDNA microarray analysis, we identified core-binding factor β (CBFβ), which together with the Runx proteins forms the heterodimeric core-binding transcription complex CBF, as a novel JunB target gene. In line with our findings, expression of the CBF target MMP-13 was impaired in JunB-deficient ECs. Reintroduction of CBFβ into JunB-deficient ECs rescued the tube formation defect and MMP-13 expression, indicating an important role for CBFβ in EC morphogenesis. |
format | Text |
id | pubmed-2064707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20647072007-11-29 JunB is required for endothelial cell morphogenesis by regulating core-binding factor β Licht, Alexander H. Pein, Oliver T. Florin, Lore Hartenstein, Bettina Reuter, Hendrik Arnold, Bernd Lichter, Peter Angel, Peter Schorpp-Kistner, Marina J Cell Biol Research Articles The molecular mechanism triggering the organization of endothelial cells (ECs) in multicellular tubules is mechanistically still poorly understood. We demonstrate that cell-autonomous endothelial functions of the AP-1 subunit JunB are required for proper endothelial morphogenesis both in vivo in mouse embryos with endothelial-specific ablation of JunB and in in vitro angiogenesis models. By cDNA microarray analysis, we identified core-binding factor β (CBFβ), which together with the Runx proteins forms the heterodimeric core-binding transcription complex CBF, as a novel JunB target gene. In line with our findings, expression of the CBF target MMP-13 was impaired in JunB-deficient ECs. Reintroduction of CBFβ into JunB-deficient ECs rescued the tube formation defect and MMP-13 expression, indicating an important role for CBFβ in EC morphogenesis. The Rockefeller University Press 2006-12-18 /pmc/articles/PMC2064707/ /pubmed/17158955 http://dx.doi.org/10.1083/jcb.200605149 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Licht, Alexander H. Pein, Oliver T. Florin, Lore Hartenstein, Bettina Reuter, Hendrik Arnold, Bernd Lichter, Peter Angel, Peter Schorpp-Kistner, Marina JunB is required for endothelial cell morphogenesis by regulating core-binding factor β |
title | JunB is required for endothelial cell morphogenesis by regulating core-binding factor β |
title_full | JunB is required for endothelial cell morphogenesis by regulating core-binding factor β |
title_fullStr | JunB is required for endothelial cell morphogenesis by regulating core-binding factor β |
title_full_unstemmed | JunB is required for endothelial cell morphogenesis by regulating core-binding factor β |
title_short | JunB is required for endothelial cell morphogenesis by regulating core-binding factor β |
title_sort | junb is required for endothelial cell morphogenesis by regulating core-binding factor β |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064707/ https://www.ncbi.nlm.nih.gov/pubmed/17158955 http://dx.doi.org/10.1083/jcb.200605149 |
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