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The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
Sensory axonal projections into the spinal cord display a highly stereotyped pattern of T- or Y-shaped axon bifurcation at the dorsal root entry zone (DREZ). Here, we provide evidence that embryonic mice with an inactive receptor guanylyl cyclase Npr2 or deficient for cyclic guanosine monophosphate–...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064768/ https://www.ncbi.nlm.nih.gov/pubmed/17954614 http://dx.doi.org/10.1083/jcb.200707176 |
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author | Schmidt, Hannes Stonkute, Agne Jüttner, René Schäffer, Susanne Buttgereit, Jens Feil, Robert Hofmann, Franz Rathjen, Fritz G. |
author_facet | Schmidt, Hannes Stonkute, Agne Jüttner, René Schäffer, Susanne Buttgereit, Jens Feil, Robert Hofmann, Franz Rathjen, Fritz G. |
author_sort | Schmidt, Hannes |
collection | PubMed |
description | Sensory axonal projections into the spinal cord display a highly stereotyped pattern of T- or Y-shaped axon bifurcation at the dorsal root entry zone (DREZ). Here, we provide evidence that embryonic mice with an inactive receptor guanylyl cyclase Npr2 or deficient for cyclic guanosine monophosphate–dependent protein kinase I (cGKI) lack the bifurcation of sensory axons at the DREZ, i.e., the ingrowing axon either turns rostrally or caudally. This bifurcation error is maintained to mature stages. In contrast, interstitial branching of collaterals from primary stem axons remains unaffected, indicating that bifurcation and interstitial branching are processes regulated by a distinct molecular mechanism. At a functional level, the distorted axonal branching at the DREZ is accompanied by reduced synaptic input, as revealed by patch clamp recordings of neurons in the superficial layers of the spinal cord. Hence, our data demonstrate that Npr2 and cGKI are essential constituents of the signaling pathway underlying axonal bifurcation at the DREZ and neuronal connectivity in the dorsal spinal cord. |
format | Text |
id | pubmed-2064768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20647682008-04-22 The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord Schmidt, Hannes Stonkute, Agne Jüttner, René Schäffer, Susanne Buttgereit, Jens Feil, Robert Hofmann, Franz Rathjen, Fritz G. J Cell Biol Research Articles Sensory axonal projections into the spinal cord display a highly stereotyped pattern of T- or Y-shaped axon bifurcation at the dorsal root entry zone (DREZ). Here, we provide evidence that embryonic mice with an inactive receptor guanylyl cyclase Npr2 or deficient for cyclic guanosine monophosphate–dependent protein kinase I (cGKI) lack the bifurcation of sensory axons at the DREZ, i.e., the ingrowing axon either turns rostrally or caudally. This bifurcation error is maintained to mature stages. In contrast, interstitial branching of collaterals from primary stem axons remains unaffected, indicating that bifurcation and interstitial branching are processes regulated by a distinct molecular mechanism. At a functional level, the distorted axonal branching at the DREZ is accompanied by reduced synaptic input, as revealed by patch clamp recordings of neurons in the superficial layers of the spinal cord. Hence, our data demonstrate that Npr2 and cGKI are essential constituents of the signaling pathway underlying axonal bifurcation at the DREZ and neuronal connectivity in the dorsal spinal cord. The Rockefeller University Press 2007-10-22 /pmc/articles/PMC2064768/ /pubmed/17954614 http://dx.doi.org/10.1083/jcb.200707176 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Schmidt, Hannes Stonkute, Agne Jüttner, René Schäffer, Susanne Buttgereit, Jens Feil, Robert Hofmann, Franz Rathjen, Fritz G. The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord |
title | The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord |
title_full | The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord |
title_fullStr | The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord |
title_full_unstemmed | The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord |
title_short | The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord |
title_sort | receptor guanylyl cyclase npr2 is essential for sensory axon bifurcation within the spinal cord |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064768/ https://www.ncbi.nlm.nih.gov/pubmed/17954614 http://dx.doi.org/10.1083/jcb.200707176 |
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