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The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord

Sensory axonal projections into the spinal cord display a highly stereotyped pattern of T- or Y-shaped axon bifurcation at the dorsal root entry zone (DREZ). Here, we provide evidence that embryonic mice with an inactive receptor guanylyl cyclase Npr2 or deficient for cyclic guanosine monophosphate–...

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Autores principales: Schmidt, Hannes, Stonkute, Agne, Jüttner, René, Schäffer, Susanne, Buttgereit, Jens, Feil, Robert, Hofmann, Franz, Rathjen, Fritz G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064768/
https://www.ncbi.nlm.nih.gov/pubmed/17954614
http://dx.doi.org/10.1083/jcb.200707176
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author Schmidt, Hannes
Stonkute, Agne
Jüttner, René
Schäffer, Susanne
Buttgereit, Jens
Feil, Robert
Hofmann, Franz
Rathjen, Fritz G.
author_facet Schmidt, Hannes
Stonkute, Agne
Jüttner, René
Schäffer, Susanne
Buttgereit, Jens
Feil, Robert
Hofmann, Franz
Rathjen, Fritz G.
author_sort Schmidt, Hannes
collection PubMed
description Sensory axonal projections into the spinal cord display a highly stereotyped pattern of T- or Y-shaped axon bifurcation at the dorsal root entry zone (DREZ). Here, we provide evidence that embryonic mice with an inactive receptor guanylyl cyclase Npr2 or deficient for cyclic guanosine monophosphate–dependent protein kinase I (cGKI) lack the bifurcation of sensory axons at the DREZ, i.e., the ingrowing axon either turns rostrally or caudally. This bifurcation error is maintained to mature stages. In contrast, interstitial branching of collaterals from primary stem axons remains unaffected, indicating that bifurcation and interstitial branching are processes regulated by a distinct molecular mechanism. At a functional level, the distorted axonal branching at the DREZ is accompanied by reduced synaptic input, as revealed by patch clamp recordings of neurons in the superficial layers of the spinal cord. Hence, our data demonstrate that Npr2 and cGKI are essential constituents of the signaling pathway underlying axonal bifurcation at the DREZ and neuronal connectivity in the dorsal spinal cord.
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spelling pubmed-20647682008-04-22 The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord Schmidt, Hannes Stonkute, Agne Jüttner, René Schäffer, Susanne Buttgereit, Jens Feil, Robert Hofmann, Franz Rathjen, Fritz G. J Cell Biol Research Articles Sensory axonal projections into the spinal cord display a highly stereotyped pattern of T- or Y-shaped axon bifurcation at the dorsal root entry zone (DREZ). Here, we provide evidence that embryonic mice with an inactive receptor guanylyl cyclase Npr2 or deficient for cyclic guanosine monophosphate–dependent protein kinase I (cGKI) lack the bifurcation of sensory axons at the DREZ, i.e., the ingrowing axon either turns rostrally or caudally. This bifurcation error is maintained to mature stages. In contrast, interstitial branching of collaterals from primary stem axons remains unaffected, indicating that bifurcation and interstitial branching are processes regulated by a distinct molecular mechanism. At a functional level, the distorted axonal branching at the DREZ is accompanied by reduced synaptic input, as revealed by patch clamp recordings of neurons in the superficial layers of the spinal cord. Hence, our data demonstrate that Npr2 and cGKI are essential constituents of the signaling pathway underlying axonal bifurcation at the DREZ and neuronal connectivity in the dorsal spinal cord. The Rockefeller University Press 2007-10-22 /pmc/articles/PMC2064768/ /pubmed/17954614 http://dx.doi.org/10.1083/jcb.200707176 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Schmidt, Hannes
Stonkute, Agne
Jüttner, René
Schäffer, Susanne
Buttgereit, Jens
Feil, Robert
Hofmann, Franz
Rathjen, Fritz G.
The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
title The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
title_full The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
title_fullStr The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
title_full_unstemmed The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
title_short The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord
title_sort receptor guanylyl cyclase npr2 is essential for sensory axon bifurcation within the spinal cord
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064768/
https://www.ncbi.nlm.nih.gov/pubmed/17954614
http://dx.doi.org/10.1083/jcb.200707176
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