Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.

The metastasis-suppressor nme gene (also called nm23), first identified in murine melanoma cells, exists as two forms in human: nme1 and nme2. However, only the lack of expression of nme1 has been related to distant metastasis appearance in human breast cancer. The aim of this work was first to rais...

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Autores principales: Toulas, C., Mihura, J., de Balincourt, C., Marques, B., Marek, E., Soula, G., Roche, H., Favre, G.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074342/
https://www.ncbi.nlm.nih.gov/pubmed/8605098
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author Toulas, C.
Mihura, J.
de Balincourt, C.
Marques, B.
Marek, E.
Soula, G.
Roche, H.
Favre, G.
author_facet Toulas, C.
Mihura, J.
de Balincourt, C.
Marques, B.
Marek, E.
Soula, G.
Roche, H.
Favre, G.
author_sort Toulas, C.
collection PubMed
description The metastasis-suppressor nme gene (also called nm23), first identified in murine melanoma cells, exists as two forms in human: nme1 and nme2. However, only the lack of expression of nme1 has been related to distant metastasis appearance in human breast cancer. The aim of this work was first to raise specific antibodies to allow the analysis of Nme1 and then, with this specific tool, to evaluate the predictive value of Nme1 detection in cytosolic extracts of human breast tumours. We obtained a hen antibody that specifically reacts with Nme1 without any cross-reaction with Nme2. We analysed the expression of the protein in 59 human breast tumours and found a significant relationship between this expression and oestrogen receptor status (P<0.001). Moreover, Nme1 expression is related to metastasis-free survival (P<0.001) and survival of patients (P<0.001). The determination of Nme1 expression in primary tumours using our antibody should be an interesting predictive test of the metastasis for clinical investigations. IMAGES:
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spelling pubmed-20743422009-09-10 Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody. Toulas, C. Mihura, J. de Balincourt, C. Marques, B. Marek, E. Soula, G. Roche, H. Favre, G. Br J Cancer Research Article The metastasis-suppressor nme gene (also called nm23), first identified in murine melanoma cells, exists as two forms in human: nme1 and nme2. However, only the lack of expression of nme1 has been related to distant metastasis appearance in human breast cancer. The aim of this work was first to raise specific antibodies to allow the analysis of Nme1 and then, with this specific tool, to evaluate the predictive value of Nme1 detection in cytosolic extracts of human breast tumours. We obtained a hen antibody that specifically reacts with Nme1 without any cross-reaction with Nme2. We analysed the expression of the protein in 59 human breast tumours and found a significant relationship between this expression and oestrogen receptor status (P<0.001). Moreover, Nme1 expression is related to metastasis-free survival (P<0.001) and survival of patients (P<0.001). The determination of Nme1 expression in primary tumours using our antibody should be an interesting predictive test of the metastasis for clinical investigations. IMAGES: Nature Publishing Group 1996-03 /pmc/articles/PMC2074342/ /pubmed/8605098 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Toulas, C.
Mihura, J.
de Balincourt, C.
Marques, B.
Marek, E.
Soula, G.
Roche, H.
Favre, G.
Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.
title Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.
title_full Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.
title_fullStr Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.
title_full_unstemmed Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.
title_short Potential prognostic value in human breast cancer of cytosolic Nme1 protein detection using an original hen specific antibody.
title_sort potential prognostic value in human breast cancer of cytosolic nme1 protein detection using an original hen specific antibody.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074342/
https://www.ncbi.nlm.nih.gov/pubmed/8605098
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