Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.

The expression of thymidine kinase--an enzyme of the DNA precursor pathway--is strictly regulated during the normal cellular cycle, but is much higher and permanently expressed in malignant growing cells. We used this fact to detect neoplastic cells in samples freshly taken from leukaemia patients a...

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Autores principales: Hengstschläger, M., Pfeilstöcker, M., Wawra, E.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074528/
https://www.ncbi.nlm.nih.gov/pubmed/8630285
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author Hengstschläger, M.
Pfeilstöcker, M.
Wawra, E.
author_facet Hengstschläger, M.
Pfeilstöcker, M.
Wawra, E.
author_sort Hengstschläger, M.
collection PubMed
description The expression of thymidine kinase--an enzyme of the DNA precursor pathway--is strictly regulated during the normal cellular cycle, but is much higher and permanently expressed in malignant growing cells. We used this fact to detect neoplastic cells in samples freshly taken from leukaemia patients and kept frozen in liquid nitrogen until analysis. Using a new cytofluorometric assay for thymidine kinase in single cells, we were able to identify leukaemic cells in a surplus of normal ones. Our results demonstrate the benefits of this assay for leukaemia diagnosis.
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spelling pubmed-20745282009-09-10 Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay. Hengstschläger, M. Pfeilstöcker, M. Wawra, E. Br J Cancer Research Article The expression of thymidine kinase--an enzyme of the DNA precursor pathway--is strictly regulated during the normal cellular cycle, but is much higher and permanently expressed in malignant growing cells. We used this fact to detect neoplastic cells in samples freshly taken from leukaemia patients and kept frozen in liquid nitrogen until analysis. Using a new cytofluorometric assay for thymidine kinase in single cells, we were able to identify leukaemic cells in a surplus of normal ones. Our results demonstrate the benefits of this assay for leukaemia diagnosis. Nature Publishing Group 1996-05 /pmc/articles/PMC2074528/ /pubmed/8630285 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Hengstschläger, M.
Pfeilstöcker, M.
Wawra, E.
Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
title Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
title_full Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
title_fullStr Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
title_full_unstemmed Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
title_short Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
title_sort identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074528/
https://www.ncbi.nlm.nih.gov/pubmed/8630285
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AT wawrae identificationofleukaemiccellsinbonemarrowandbloodsamplesbyanewcytofluorometricassay

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