Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.

Neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of MAb A7 (chA7Fab) directed against human pancreatic carcinoma. The anti-tumour effect of chA7Fab-NCS was tested in a nude mouse model on pancreatic carcinoma and compared with A7-NCS or NCS alone. The anti-tumour eff...

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Autores principales: Otsuji, E., Yamaguchi, T., Tsuruta, H., Yata, Y., Nishi, H., Okamoto, K., Taniguchi, K., Kato, M., Kotani, T., Kitamura, K., Takahashi, T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074529/
https://www.ncbi.nlm.nih.gov/pubmed/8630275
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author Otsuji, E.
Yamaguchi, T.
Tsuruta, H.
Yata, Y.
Nishi, H.
Okamoto, K.
Taniguchi, K.
Kato, M.
Kotani, T.
Kitamura, K.
Takahashi, T.
author_facet Otsuji, E.
Yamaguchi, T.
Tsuruta, H.
Yata, Y.
Nishi, H.
Okamoto, K.
Taniguchi, K.
Kato, M.
Kotani, T.
Kitamura, K.
Takahashi, T.
author_sort Otsuji, E.
collection PubMed
description Neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of MAb A7 (chA7Fab) directed against human pancreatic carcinoma. The anti-tumour effect of chA7Fab-NCS was tested in a nude mouse model on pancreatic carcinoma and compared with A7-NCS or NCS alone. The anti-tumour effect of chA7Fab-NCS increased in a dose-dependent manner and was significantly greater than either A7-NCS or NCS. Tumour growth was completely suppressed after the administration of chA7Fab-NCS. An enzyme-linked immunosorbent assay with rabbit anti-mouse immunoglobulin was performed to examine the antigenicity of chA7Fab. ChA7Fab had less reactivity with rabbit anti-mouse immunoglobulin than either whole antibody A7 or murine Fab fragments of A7. Thus, chA7Fab-NCS can inhibit human pancreatic cancer growth in an animal and may be useful for targeting chemotherapy to pancreatic cancer in humans.
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spelling pubmed-20745292009-09-10 Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts. Otsuji, E. Yamaguchi, T. Tsuruta, H. Yata, Y. Nishi, H. Okamoto, K. Taniguchi, K. Kato, M. Kotani, T. Kitamura, K. Takahashi, T. Br J Cancer Research Article Neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of MAb A7 (chA7Fab) directed against human pancreatic carcinoma. The anti-tumour effect of chA7Fab-NCS was tested in a nude mouse model on pancreatic carcinoma and compared with A7-NCS or NCS alone. The anti-tumour effect of chA7Fab-NCS increased in a dose-dependent manner and was significantly greater than either A7-NCS or NCS. Tumour growth was completely suppressed after the administration of chA7Fab-NCS. An enzyme-linked immunosorbent assay with rabbit anti-mouse immunoglobulin was performed to examine the antigenicity of chA7Fab. ChA7Fab had less reactivity with rabbit anti-mouse immunoglobulin than either whole antibody A7 or murine Fab fragments of A7. Thus, chA7Fab-NCS can inhibit human pancreatic cancer growth in an animal and may be useful for targeting chemotherapy to pancreatic cancer in humans. Nature Publishing Group 1996-05 /pmc/articles/PMC2074529/ /pubmed/8630275 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Otsuji, E.
Yamaguchi, T.
Tsuruta, H.
Yata, Y.
Nishi, H.
Okamoto, K.
Taniguchi, K.
Kato, M.
Kotani, T.
Kitamura, K.
Takahashi, T.
Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
title Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
title_full Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
title_fullStr Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
title_full_unstemmed Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
title_short Effects of neocarzinostatin-chimeric Fab conjugates on the growth of human pancreatic carcinoma xenografts.
title_sort effects of neocarzinostatin-chimeric fab conjugates on the growth of human pancreatic carcinoma xenografts.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074529/
https://www.ncbi.nlm.nih.gov/pubmed/8630275
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