Rarity of microsatellite alterations in acute myeloid leukaemia.

We have analysed samples from 20 patients with acute myeloid leukaemia for microsatellite alterations by comparing constitutional DNA and DNA from leukaemic samples. Twelve microsatellites were amplified by PCR and investigated for novel bands, indicative of microsatellite instability, or for loss o...

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Detalles Bibliográficos
Autores principales: Sill, H., Goldman, J. M., Cross, N. C.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074583/
https://www.ncbi.nlm.nih.gov/pubmed/8688331
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author Sill, H.
Goldman, J. M.
Cross, N. C.
author_facet Sill, H.
Goldman, J. M.
Cross, N. C.
author_sort Sill, H.
collection PubMed
description We have analysed samples from 20 patients with acute myeloid leukaemia for microsatellite alterations by comparing constitutional DNA and DNA from leukaemic samples. Twelve microsatellites were amplified by PCR and investigated for novel bands, indicative of microsatellite instability, or for loss of heterozygosity. Out of 215 paired amplifications, no additional bands were observed at any locus in any of the samples analysed and loss of heterozygosity was found only as four loci from three patients. These results suggest that microsatellite alterations are very uncommon in acute myeloid leukaemia. IMAGES:
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spelling pubmed-20745832009-09-10 Rarity of microsatellite alterations in acute myeloid leukaemia. Sill, H. Goldman, J. M. Cross, N. C. Br J Cancer Research Article We have analysed samples from 20 patients with acute myeloid leukaemia for microsatellite alterations by comparing constitutional DNA and DNA from leukaemic samples. Twelve microsatellites were amplified by PCR and investigated for novel bands, indicative of microsatellite instability, or for loss of heterozygosity. Out of 215 paired amplifications, no additional bands were observed at any locus in any of the samples analysed and loss of heterozygosity was found only as four loci from three patients. These results suggest that microsatellite alterations are very uncommon in acute myeloid leukaemia. IMAGES: Nature Publishing Group 1996-07 /pmc/articles/PMC2074583/ /pubmed/8688331 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Sill, H.
Goldman, J. M.
Cross, N. C.
Rarity of microsatellite alterations in acute myeloid leukaemia.
title Rarity of microsatellite alterations in acute myeloid leukaemia.
title_full Rarity of microsatellite alterations in acute myeloid leukaemia.
title_fullStr Rarity of microsatellite alterations in acute myeloid leukaemia.
title_full_unstemmed Rarity of microsatellite alterations in acute myeloid leukaemia.
title_short Rarity of microsatellite alterations in acute myeloid leukaemia.
title_sort rarity of microsatellite alterations in acute myeloid leukaemia.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074583/
https://www.ncbi.nlm.nih.gov/pubmed/8688331
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AT crossnc rarityofmicrosatellitealterationsinacutemyeloidleukaemia

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