DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.

In vivo DNA damage and repair was induced by nicotinamide (NAM) in adenotype 12 virus-induced mouse sarcoma A12B3 and sarcoma F inoculated into CBA mice. DNA damage, NAM and NAD concentrations were measured after in vivo exposure to NAM, in tumours and spleens by alkaline elution and by HPLC analysi...

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Autores principales: Olsson, A. R., Sheng, Y., Pero, R. W., Chaplin, D. J., Horsman, M. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1996
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074637/
https://www.ncbi.nlm.nih.gov/pubmed/8695350
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author Olsson, A. R.
Sheng, Y.
Pero, R. W.
Chaplin, D. J.
Horsman, M. R.
author_facet Olsson, A. R.
Sheng, Y.
Pero, R. W.
Chaplin, D. J.
Horsman, M. R.
author_sort Olsson, A. R.
collection PubMed
description In vivo DNA damage and repair was induced by nicotinamide (NAM) in adenotype 12 virus-induced mouse sarcoma A12B3 and sarcoma F inoculated into CBA mice. DNA damage, NAM and NAD concentrations were measured after in vivo exposure to NAM, in tumours and spleens by alkaline elution and by HPLC analysis. Our results indicate that NAM between 100-1000 mg kg-1 causes a high level of in vivo DNA strand breaks in tumours and normal tissues in mice bearing the immunogenic sarcoma A12B3 but not in the non-immunogenic sarcoma F. The repair process was also delayed by the NAM treatment probably owing to inhibition of the DNA repair enzyme, poly(ADP-ribose)polymerase, as evidenced by accumulation of NAM and NAD. These data are consistent with NAM having a mechanism of action as a radiosensitiser at least in part by DNA repair inhibition. In addition, it should also be considered that high doses of NAM might cause considerable complications to normal tissue in tumour-bearing individuals.
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spelling pubmed-20746372009-09-10 DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide. Olsson, A. R. Sheng, Y. Pero, R. W. Chaplin, D. J. Horsman, M. R. Br J Cancer Research Article In vivo DNA damage and repair was induced by nicotinamide (NAM) in adenotype 12 virus-induced mouse sarcoma A12B3 and sarcoma F inoculated into CBA mice. DNA damage, NAM and NAD concentrations were measured after in vivo exposure to NAM, in tumours and spleens by alkaline elution and by HPLC analysis. Our results indicate that NAM between 100-1000 mg kg-1 causes a high level of in vivo DNA strand breaks in tumours and normal tissues in mice bearing the immunogenic sarcoma A12B3 but not in the non-immunogenic sarcoma F. The repair process was also delayed by the NAM treatment probably owing to inhibition of the DNA repair enzyme, poly(ADP-ribose)polymerase, as evidenced by accumulation of NAM and NAD. These data are consistent with NAM having a mechanism of action as a radiosensitiser at least in part by DNA repair inhibition. In addition, it should also be considered that high doses of NAM might cause considerable complications to normal tissue in tumour-bearing individuals. Nature Publishing Group 1996-08 /pmc/articles/PMC2074637/ /pubmed/8695350 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Olsson, A. R.
Sheng, Y.
Pero, R. W.
Chaplin, D. J.
Horsman, M. R.
DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
title DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
title_full DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
title_fullStr DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
title_full_unstemmed DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
title_short DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
title_sort dna damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2074637/
https://www.ncbi.nlm.nih.gov/pubmed/8695350
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