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Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat

There is a considerable gap between investigating the dynamics of single neurons and the computational aspects of neural networks. A growing number of studies have attempted to overcome this gap using the excitation in brain slices elicited by various chemical manipulations of the bath solution. How...

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Detalles Bibliográficos
Autores principales: Bar-Yehuda, Dan, Korngreen, Alon
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075161/
https://www.ncbi.nlm.nih.gov/pubmed/18030343
http://dx.doi.org/10.1371/journal.pone.0001209
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author Bar-Yehuda, Dan
Korngreen, Alon
author_facet Bar-Yehuda, Dan
Korngreen, Alon
author_sort Bar-Yehuda, Dan
collection PubMed
description There is a considerable gap between investigating the dynamics of single neurons and the computational aspects of neural networks. A growing number of studies have attempted to overcome this gap using the excitation in brain slices elicited by various chemical manipulations of the bath solution. However, there has been no quantitative study on the effects of these manipulations on the cellular and network factors controlling excitability. Using the whole-cell configuration of the patch-clamp technique we recorded the membrane potential from the soma of layer 5 pyramidal neurons in acute brain slices from the somatosensory cortex of young rats at 22°C and 35°C. Using blockers of synaptic transmission, we show distinct changes in cellular properties following modification of the ionic composition of the artificial cerebrospinal fluid (ACSF). Thus both cellular and network changes may contribute to the observed effects of slice excitation solutions on the physiology of single neurons. Furthermore, our data suggest that the difference in the ionic composition of current standard ACSF from that of CSF measured in vivo cause ACSF to depress network activity in acute brain slices. This may affect outcomes of experiments investigating biophysical and physiological properties of neurons in such preparations. Our results strongly advocate the necessity of redesigning experiments routinely carried out in the quiescent acute brain slice preparation.
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spelling pubmed-20751612007-11-21 Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat Bar-Yehuda, Dan Korngreen, Alon PLoS One Research Article There is a considerable gap between investigating the dynamics of single neurons and the computational aspects of neural networks. A growing number of studies have attempted to overcome this gap using the excitation in brain slices elicited by various chemical manipulations of the bath solution. However, there has been no quantitative study on the effects of these manipulations on the cellular and network factors controlling excitability. Using the whole-cell configuration of the patch-clamp technique we recorded the membrane potential from the soma of layer 5 pyramidal neurons in acute brain slices from the somatosensory cortex of young rats at 22°C and 35°C. Using blockers of synaptic transmission, we show distinct changes in cellular properties following modification of the ionic composition of the artificial cerebrospinal fluid (ACSF). Thus both cellular and network changes may contribute to the observed effects of slice excitation solutions on the physiology of single neurons. Furthermore, our data suggest that the difference in the ionic composition of current standard ACSF from that of CSF measured in vivo cause ACSF to depress network activity in acute brain slices. This may affect outcomes of experiments investigating biophysical and physiological properties of neurons in such preparations. Our results strongly advocate the necessity of redesigning experiments routinely carried out in the quiescent acute brain slice preparation. Public Library of Science 2007-11-21 /pmc/articles/PMC2075161/ /pubmed/18030343 http://dx.doi.org/10.1371/journal.pone.0001209 Text en Bar-Yehuda, Korngreen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bar-Yehuda, Dan
Korngreen, Alon
Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat
title Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat
title_full Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat
title_fullStr Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat
title_full_unstemmed Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat
title_short Cellular and Network Contributions to Excitability of Layer 5 Neocortical Pyramidal Neurons in the Rat
title_sort cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075161/
https://www.ncbi.nlm.nih.gov/pubmed/18030343
http://dx.doi.org/10.1371/journal.pone.0001209
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