Cargando…
Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness.
The nm23-H1 gene is a putative metastasis-suppressor gene encoding a 17 kDa protein with nucleoside diphosphate kinase activity. Expression of nm23-H1/NDPK-A correlates inversely with the metastasising potential of some human tumours and experimental animal cells. No nm23 expression studies exist fo...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1996
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077220/ https://www.ncbi.nlm.nih.gov/pubmed/8956779 |
_version_ | 1782138108243869696 |
---|---|
author | Aryee, D. N. Simonitsch, I. Mosberger, I. Kos, K. Mann, G. Schlögl, E. Pötschger, U. Gadner, H. Radaszkiewicz, T. Kovar, H. |
author_facet | Aryee, D. N. Simonitsch, I. Mosberger, I. Kos, K. Mann, G. Schlögl, E. Pötschger, U. Gadner, H. Radaszkiewicz, T. Kovar, H. |
author_sort | Aryee, D. N. |
collection | PubMed |
description | The nm23-H1 gene is a putative metastasis-suppressor gene encoding a 17 kDa protein with nucleoside diphosphate kinase activity. Expression of nm23-H1/NDPK-A correlates inversely with the metastasising potential of some human tumours and experimental animal cells. No nm23 expression studies exist for human malignant lymphomas so far. In this study, we examined nm23-H1 expression by Northern and immunohistochemical analysis in 106 primary lymphoma samples from patients with Hodgkin's disease (HD) (n = 15), high-grade non-Hodgkin's lymphoma (NHL) from different lineages (n = 71) and low-grade NHL (n = 20). Both inter- and intra-subtype variations in nm23-H1/NDPK-A expression levels were demonstrated by all disease subtypes. Besides this heterogeneity, a general trend towards highly malignant samples expressing higher nm23-H1/NDPK-A, levels than the low-grade lymphomas was observed. Both adult and childhood HD and high-grade NHL samples exhibited significantly higher NDPK-A expression than the low-grade NHL found only in adults. High nm23-H1/NDPK-A levels in lymphoma samples did not always reflect proliferative activity of tumour cells as monitored by Ki-67 antigen staining. Fifty samples were further investigated for possible mutations in the nm23-H1 coding sequence by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and single-strand conformation polymorphism (SSCP) analysis. No mutation was found by this screening. Our results suggest a role for nm23-H1 expression in the disease aggressiveness of lymphomas. IMAGES: |
format | Text |
id | pubmed-2077220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20772202009-09-10 Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. Aryee, D. N. Simonitsch, I. Mosberger, I. Kos, K. Mann, G. Schlögl, E. Pötschger, U. Gadner, H. Radaszkiewicz, T. Kovar, H. Br J Cancer Research Article The nm23-H1 gene is a putative metastasis-suppressor gene encoding a 17 kDa protein with nucleoside diphosphate kinase activity. Expression of nm23-H1/NDPK-A correlates inversely with the metastasising potential of some human tumours and experimental animal cells. No nm23 expression studies exist for human malignant lymphomas so far. In this study, we examined nm23-H1 expression by Northern and immunohistochemical analysis in 106 primary lymphoma samples from patients with Hodgkin's disease (HD) (n = 15), high-grade non-Hodgkin's lymphoma (NHL) from different lineages (n = 71) and low-grade NHL (n = 20). Both inter- and intra-subtype variations in nm23-H1/NDPK-A expression levels were demonstrated by all disease subtypes. Besides this heterogeneity, a general trend towards highly malignant samples expressing higher nm23-H1/NDPK-A, levels than the low-grade lymphomas was observed. Both adult and childhood HD and high-grade NHL samples exhibited significantly higher NDPK-A expression than the low-grade NHL found only in adults. High nm23-H1/NDPK-A levels in lymphoma samples did not always reflect proliferative activity of tumour cells as monitored by Ki-67 antigen staining. Fifty samples were further investigated for possible mutations in the nm23-H1 coding sequence by means of reverse transcriptase-polymerase chain reaction (RT-PCR) and single-strand conformation polymorphism (SSCP) analysis. No mutation was found by this screening. Our results suggest a role for nm23-H1 expression in the disease aggressiveness of lymphomas. IMAGES: Nature Publishing Group 1996-12 /pmc/articles/PMC2077220/ /pubmed/8956779 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Aryee, D. N. Simonitsch, I. Mosberger, I. Kos, K. Mann, G. Schlögl, E. Pötschger, U. Gadner, H. Radaszkiewicz, T. Kovar, H. Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. |
title | Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. |
title_full | Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. |
title_fullStr | Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. |
title_full_unstemmed | Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. |
title_short | Variability of nm23-H1/NDPK-A expression in human lymphomas and its relation to tumour aggressiveness. |
title_sort | variability of nm23-h1/ndpk-a expression in human lymphomas and its relation to tumour aggressiveness. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2077220/ https://www.ncbi.nlm.nih.gov/pubmed/8956779 |
work_keys_str_mv | AT aryeedn variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT simonitschi variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT mosbergeri variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT kosk variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT manng variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT schlogle variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT potschgeru variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT gadnerh variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT radaszkiewiczt variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness AT kovarh variabilityofnm23h1ndpkaexpressioninhumanlymphomasanditsrelationtotumouraggressiveness |