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Over-expression of 14-3-3zeta is an early event in oral cancer
BACKGROUND: The functional and clinical significance of 14-3-3 proteins in human cancers remain largely undetermined. Earlier, we have reported differential expression of 14-3-3ζ mRNA in oral squamous cell carcinoma (OSCC) by differential display. METHODS: The clinical relevance of 14-3-3ζ protein i...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2082039/ https://www.ncbi.nlm.nih.gov/pubmed/17764575 http://dx.doi.org/10.1186/1471-2407-7-169 |
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author | Matta, Ajay Bahadur, Sudhir Duggal, Ritu Gupta, Siddhartha D Ralhan, Ranju |
author_facet | Matta, Ajay Bahadur, Sudhir Duggal, Ritu Gupta, Siddhartha D Ralhan, Ranju |
author_sort | Matta, Ajay |
collection | PubMed |
description | BACKGROUND: The functional and clinical significance of 14-3-3 proteins in human cancers remain largely undetermined. Earlier, we have reported differential expression of 14-3-3ζ mRNA in oral squamous cell carcinoma (OSCC) by differential display. METHODS: The clinical relevance of 14-3-3ζ protein in oral tumorigenesis was determined by immunohistochemistry in paraffin embedded sections of oral pre-malignant lesions (OPLs), OSCCs and histologically normal oral tissues and corroborated by Western Blotting. Co-immunoprecipitation assays were carried out to determine its association with NFκB, β-catenin and Bcl-2. RESULTS: Intense immunostaining of 14-3-3ζ protein was observed in 61/89 (69%) OPLs and 95/120 (79%) OSCCs. Immunohistochemistry showed significant increase in expression of 14-3-3ζ protein from normal mucosa to OPLs to OSCCs (p(trend )< 0.001). Significant increase in expression of 14-3-3ζ protein was observed as early as in hyperplasia (p = 0.009), with further elevation in moderate and severe dysplasia, that was sustained in OSCCs. These findings were validated by Western blotting. Using Co-immunoprecipitation, we demonstrated that 14-3-3ζ protein binds to NFκB, β-catenin and Bcl-2, suggesting its involvement in cellular signaling, leading to proliferation of oral cancer cells. CONCLUSION: Our findings suggest that over-expression of 14-3-3ζ is an early event in oral tumorigenesis and may have an important role in its development and progression. Thus, 14-3-3ζ may serve as an important molecular target for designing novel therapy for oral cancer. |
format | Text |
id | pubmed-2082039 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-20820392007-11-20 Over-expression of 14-3-3zeta is an early event in oral cancer Matta, Ajay Bahadur, Sudhir Duggal, Ritu Gupta, Siddhartha D Ralhan, Ranju BMC Cancer Research Article BACKGROUND: The functional and clinical significance of 14-3-3 proteins in human cancers remain largely undetermined. Earlier, we have reported differential expression of 14-3-3ζ mRNA in oral squamous cell carcinoma (OSCC) by differential display. METHODS: The clinical relevance of 14-3-3ζ protein in oral tumorigenesis was determined by immunohistochemistry in paraffin embedded sections of oral pre-malignant lesions (OPLs), OSCCs and histologically normal oral tissues and corroborated by Western Blotting. Co-immunoprecipitation assays were carried out to determine its association with NFκB, β-catenin and Bcl-2. RESULTS: Intense immunostaining of 14-3-3ζ protein was observed in 61/89 (69%) OPLs and 95/120 (79%) OSCCs. Immunohistochemistry showed significant increase in expression of 14-3-3ζ protein from normal mucosa to OPLs to OSCCs (p(trend )< 0.001). Significant increase in expression of 14-3-3ζ protein was observed as early as in hyperplasia (p = 0.009), with further elevation in moderate and severe dysplasia, that was sustained in OSCCs. These findings were validated by Western blotting. Using Co-immunoprecipitation, we demonstrated that 14-3-3ζ protein binds to NFκB, β-catenin and Bcl-2, suggesting its involvement in cellular signaling, leading to proliferation of oral cancer cells. CONCLUSION: Our findings suggest that over-expression of 14-3-3ζ is an early event in oral tumorigenesis and may have an important role in its development and progression. Thus, 14-3-3ζ may serve as an important molecular target for designing novel therapy for oral cancer. BioMed Central 2007-09-02 /pmc/articles/PMC2082039/ /pubmed/17764575 http://dx.doi.org/10.1186/1471-2407-7-169 Text en Copyright © 2007 Matta et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Matta, Ajay Bahadur, Sudhir Duggal, Ritu Gupta, Siddhartha D Ralhan, Ranju Over-expression of 14-3-3zeta is an early event in oral cancer |
title | Over-expression of 14-3-3zeta is an early event in oral cancer |
title_full | Over-expression of 14-3-3zeta is an early event in oral cancer |
title_fullStr | Over-expression of 14-3-3zeta is an early event in oral cancer |
title_full_unstemmed | Over-expression of 14-3-3zeta is an early event in oral cancer |
title_short | Over-expression of 14-3-3zeta is an early event in oral cancer |
title_sort | over-expression of 14-3-3zeta is an early event in oral cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2082039/ https://www.ncbi.nlm.nih.gov/pubmed/17764575 http://dx.doi.org/10.1186/1471-2407-7-169 |
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