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Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote

Mitochondrial ribosomal RNAs (mtrRNAs) have been reported to translocate extra-mitochondrially and localize to the germ cell determinant of oocytes and zygotes in some metazoa except mammals. To address whether the mtrRNAs also localize in the mammals, expression and distribution of mitochondrion-en...

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Autores principales: Ninomiya, Youichirou, Ichinose, Shizuko
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2082410/
https://www.ncbi.nlm.nih.gov/pubmed/18043748
http://dx.doi.org/10.1371/journal.pone.0001241
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author Ninomiya, Youichirou
Ichinose, Shizuko
author_facet Ninomiya, Youichirou
Ichinose, Shizuko
author_sort Ninomiya, Youichirou
collection PubMed
description Mitochondrial ribosomal RNAs (mtrRNAs) have been reported to translocate extra-mitochondrially and localize to the germ cell determinant of oocytes and zygotes in some metazoa except mammals. To address whether the mtrRNAs also localize in the mammals, expression and distribution of mitochondrion-encoded RNAs in the mouse oocytes and zygotes was examined by whole-mount in situ hybridization (ISH). Both 12S and 16S rRNAs were predominantly distributed in the animal hemisphere of the mature oocyte. This distribution pattern was rearranged toward the second polar body in zygotes after fertilization. The amount of mtrRNAs decreased around first cleavage, remained low during second cleavage and increased after third cleavage. Staining intensity of the 12S rRNA was weaker than that of the 16S rRNA throughout the examined stages. Similar distribution dynamics of the 16S rRNA was observed in strontium-activated haploid parthenotes, suggesting the distribution rearrangement does not require a component from sperm. The distribution of 16S rRNAs did not coincide with that of mitochondrion-specific heat shock protein 70, suggesting that the mtrRNA is translocated from mitochondria. The ISH-scanning electron microscopy confirms the extra-mitochondrial mtrRNA in the mouse oocyte. Chloramphenicol (CP) treatment of late pronuclear stage zygotes perturbed first cleavage as judged by the greater than normal disparity in size of blastomeres of 2-cell conceptuses. Two-third of the CP-treated zygotes arrested at either 2-cell or 3-cell stage even after the CP was washed out. These findings indicate that the extra-mitochondrial mtrRNAs are localized in the mouse oocyte and implicated in correct cytoplasmic segregation into blastomeres through cleavages of the zygote.
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spelling pubmed-20824102007-11-28 Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote Ninomiya, Youichirou Ichinose, Shizuko PLoS One Research Article Mitochondrial ribosomal RNAs (mtrRNAs) have been reported to translocate extra-mitochondrially and localize to the germ cell determinant of oocytes and zygotes in some metazoa except mammals. To address whether the mtrRNAs also localize in the mammals, expression and distribution of mitochondrion-encoded RNAs in the mouse oocytes and zygotes was examined by whole-mount in situ hybridization (ISH). Both 12S and 16S rRNAs were predominantly distributed in the animal hemisphere of the mature oocyte. This distribution pattern was rearranged toward the second polar body in zygotes after fertilization. The amount of mtrRNAs decreased around first cleavage, remained low during second cleavage and increased after third cleavage. Staining intensity of the 12S rRNA was weaker than that of the 16S rRNA throughout the examined stages. Similar distribution dynamics of the 16S rRNA was observed in strontium-activated haploid parthenotes, suggesting the distribution rearrangement does not require a component from sperm. The distribution of 16S rRNAs did not coincide with that of mitochondrion-specific heat shock protein 70, suggesting that the mtrRNA is translocated from mitochondria. The ISH-scanning electron microscopy confirms the extra-mitochondrial mtrRNA in the mouse oocyte. Chloramphenicol (CP) treatment of late pronuclear stage zygotes perturbed first cleavage as judged by the greater than normal disparity in size of blastomeres of 2-cell conceptuses. Two-third of the CP-treated zygotes arrested at either 2-cell or 3-cell stage even after the CP was washed out. These findings indicate that the extra-mitochondrial mtrRNAs are localized in the mouse oocyte and implicated in correct cytoplasmic segregation into blastomeres through cleavages of the zygote. Public Library of Science 2007-11-28 /pmc/articles/PMC2082410/ /pubmed/18043748 http://dx.doi.org/10.1371/journal.pone.0001241 Text en Ninomiya, Ichinose. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ninomiya, Youichirou
Ichinose, Shizuko
Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote
title Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote
title_full Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote
title_fullStr Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote
title_full_unstemmed Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote
title_short Subcellular Distribution of Mitochondrial Ribosomal RNA in the Mouse Oocyte and Zygote
title_sort subcellular distribution of mitochondrial ribosomal rna in the mouse oocyte and zygote
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2082410/
https://www.ncbi.nlm.nih.gov/pubmed/18043748
http://dx.doi.org/10.1371/journal.pone.0001241
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