Molecular epidemiology of Hepatitis B virus genotypes in Pakistan

BACKGROUND: Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population. METHODS: A total of 690 individual...

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Detalles Bibliográficos
Autores principales: Alam, Muhammad Masroor, Zaidi, Sohail Zahoor, Malik, Salman Akbar, Shaukat, Shahzad, Naeem, Asif, Sharif, Salmaan, Angez, Mehar, Butt, Javed Aslam
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2089072/
https://www.ncbi.nlm.nih.gov/pubmed/17922910
http://dx.doi.org/10.1186/1471-2334-7-115
Descripción
Sumario:BACKGROUND: Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population. METHODS: A total of 690 individuals were enrolled for HBV screening with EIA and nested PCR. Positive samples were further analyzed to determine HBV genotypes (A-F) by multiplex-PCR using type specific primers. RESULTS: 110 (15.94%) individuals were positive for HBV, including 64% males and 36% females. Out of these, 66 samples (65.34%) were classified into genotype D, 27 (26.73%) were of genotype B while 5(4.95%) had genotype A. In 3 (2.98%) samples, multiple genotypes were detected (genotype A+B; 2(1.99%) and genotypes B+D; 1(0.99%). Nine (8.18%) samples remained untyable. CONCLUSION: In Asia, genotypes B and C are the most prevalent but our study reveals that genotype D is predominant and HBV infection constitutes a significant health problem in Pakistan.

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