Cargando…

Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution

Nuclear factor 90 (NF90) is a double-stranded RNA-binding protein implicated in multiple cellular functions, but with few identified RNA partners. Using in vivo cross-linking followed by immunoprecipitation, we discovered a family of small NF90-associated RNAs (snaR). These highly structured non-cod...

Descripción completa

Detalles Bibliográficos
Autores principales: Parrott, Andrew M., Mathews, Michael B.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2094060/
https://www.ncbi.nlm.nih.gov/pubmed/17855395
http://dx.doi.org/10.1093/nar/gkm668
_version_ 1782138209589788672
author Parrott, Andrew M.
Mathews, Michael B.
author_facet Parrott, Andrew M.
Mathews, Michael B.
author_sort Parrott, Andrew M.
collection PubMed
description Nuclear factor 90 (NF90) is a double-stranded RNA-binding protein implicated in multiple cellular functions, but with few identified RNA partners. Using in vivo cross-linking followed by immunoprecipitation, we discovered a family of small NF90-associated RNAs (snaR). These highly structured non-coding RNAs of ∼117 nucleotides are expressed in immortalized human cell lines of diverse lineages. In human tissues, they are abundant in testis, with minor distribution in brain, placenta and some other organs. Two snaR subsets were isolated from human 293 cells, and additional species were found by bioinformatic analysis. Their genes often occur in multiple copies arranged in two inverted regions of tandem repeats on chromosome 19. snaR-A is transcribed by RNA polymerase III from an intragenic promoter, turns over rapidly, and shares sequence identity with Alu RNA and two potential piRNAs. It interacts with NF90's double-stranded RNA-binding motifs. snaR orthologs are present in chimpanzee but not other mammals, and include genes located in the promoter of two chorionic gonadotropin hormone genes. snaRs appear to have undergone accelerated evolution and differential expansion in the great apes.
format Text
id pubmed-2094060
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-20940602007-12-03 Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution Parrott, Andrew M. Mathews, Michael B. Nucleic Acids Res RNA Nuclear factor 90 (NF90) is a double-stranded RNA-binding protein implicated in multiple cellular functions, but with few identified RNA partners. Using in vivo cross-linking followed by immunoprecipitation, we discovered a family of small NF90-associated RNAs (snaR). These highly structured non-coding RNAs of ∼117 nucleotides are expressed in immortalized human cell lines of diverse lineages. In human tissues, they are abundant in testis, with minor distribution in brain, placenta and some other organs. Two snaR subsets were isolated from human 293 cells, and additional species were found by bioinformatic analysis. Their genes often occur in multiple copies arranged in two inverted regions of tandem repeats on chromosome 19. snaR-A is transcribed by RNA polymerase III from an intragenic promoter, turns over rapidly, and shares sequence identity with Alu RNA and two potential piRNAs. It interacts with NF90's double-stranded RNA-binding motifs. snaR orthologs are present in chimpanzee but not other mammals, and include genes located in the promoter of two chorionic gonadotropin hormone genes. snaRs appear to have undergone accelerated evolution and differential expansion in the great apes. Oxford University Press 2007-09 2007-09-13 /pmc/articles/PMC2094060/ /pubmed/17855395 http://dx.doi.org/10.1093/nar/gkm668 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Parrott, Andrew M.
Mathews, Michael B.
Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution
title Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution
title_full Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution
title_fullStr Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution
title_full_unstemmed Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution
title_short Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution
title_sort novel rapidly evolving hominid rnas bind nuclear factor 90 and display tissue-restricted distribution
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2094060/
https://www.ncbi.nlm.nih.gov/pubmed/17855395
http://dx.doi.org/10.1093/nar/gkm668
work_keys_str_mv AT parrottandrewm novelrapidlyevolvinghominidrnasbindnuclearfactor90anddisplaytissuerestricteddistribution
AT mathewsmichaelb novelrapidlyevolvinghominidrnasbindnuclearfactor90anddisplaytissuerestricteddistribution