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Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis

Expression and function of the transcription factor Sox10 is predominant in neural crest cells, its derivatives and in oligodendrocytes. To understand how Sox10 expression is regulated during development, we analysed the potential of evolutionary conserved non-coding sequences in the Sox10 genomic r...

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Detalles Bibliográficos
Autores principales: Werner, Torsten, Hammer, Alexander, Wahlbuhl, Mandy, Bösl, Michael R., Wegner, Michael
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2095789/
https://www.ncbi.nlm.nih.gov/pubmed/17897962
http://dx.doi.org/10.1093/nar/gkm727
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author Werner, Torsten
Hammer, Alexander
Wahlbuhl, Mandy
Bösl, Michael R.
Wegner, Michael
author_facet Werner, Torsten
Hammer, Alexander
Wahlbuhl, Mandy
Bösl, Michael R.
Wegner, Michael
author_sort Werner, Torsten
collection PubMed
description Expression and function of the transcription factor Sox10 is predominant in neural crest cells, its derivatives and in oligodendrocytes. To understand how Sox10 expression is regulated during development, we analysed the potential of evolutionary conserved non-coding sequences in the Sox10 genomic region to function as enhancers. By linking these sequences to a β-galactosidase marker gene under the control of a minimal promoter, five regulatory regions were identified that direct marker gene expression in transgenic mice to Sox10 expressing cell types and tissues in a defined temporal pattern. These possible enhancers of the Sox10 gene mediate Sox10 expression in the otic vesicle, in oligodendrocytes and in several neural crest derivatives including the developing peripheral nervous system and the adrenal gland. They furthermore exhibit overlapping activities and share binding sites for Sox, Lef/Tcf, Pax and AP2 transcription factors. This may explain high level and robustness of Sox10 expression during embryonic development.
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spelling pubmed-20957892007-12-07 Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis Werner, Torsten Hammer, Alexander Wahlbuhl, Mandy Bösl, Michael R. Wegner, Michael Nucleic Acids Res Genomics Expression and function of the transcription factor Sox10 is predominant in neural crest cells, its derivatives and in oligodendrocytes. To understand how Sox10 expression is regulated during development, we analysed the potential of evolutionary conserved non-coding sequences in the Sox10 genomic region to function as enhancers. By linking these sequences to a β-galactosidase marker gene under the control of a minimal promoter, five regulatory regions were identified that direct marker gene expression in transgenic mice to Sox10 expressing cell types and tissues in a defined temporal pattern. These possible enhancers of the Sox10 gene mediate Sox10 expression in the otic vesicle, in oligodendrocytes and in several neural crest derivatives including the developing peripheral nervous system and the adrenal gland. They furthermore exhibit overlapping activities and share binding sites for Sox, Lef/Tcf, Pax and AP2 transcription factors. This may explain high level and robustness of Sox10 expression during embryonic development. Oxford University Press 2007-10 2007-09-26 /pmc/articles/PMC2095789/ /pubmed/17897962 http://dx.doi.org/10.1093/nar/gkm727 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genomics
Werner, Torsten
Hammer, Alexander
Wahlbuhl, Mandy
Bösl, Michael R.
Wegner, Michael
Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis
title Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis
title_full Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis
title_fullStr Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis
title_full_unstemmed Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis
title_short Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis
title_sort multiple conserved regulatory elements with overlapping functions determine sox10 expression in mouse embryogenesis
topic Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2095789/
https://www.ncbi.nlm.nih.gov/pubmed/17897962
http://dx.doi.org/10.1093/nar/gkm727
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