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In vitro selection and characterization of cellulose-binding DNA aptamers

Many nucleic acid enzymes and aptamers have modular architectures that allow them to retain their functions when combined with other nucleotide sequences. This modular function facilitates the engineering of RNAs and DNAs that have more complex functions. We sought to create new DNA aptamers that bi...

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Detalles Bibliográficos
Autores principales: Boese, Benjamin J., Breaker, Ronald R.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2095800/
https://www.ncbi.nlm.nih.gov/pubmed/17881365
http://dx.doi.org/10.1093/nar/gkm708
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author Boese, Benjamin J.
Breaker, Ronald R.
author_facet Boese, Benjamin J.
Breaker, Ronald R.
author_sort Boese, Benjamin J.
collection PubMed
description Many nucleic acid enzymes and aptamers have modular architectures that allow them to retain their functions when combined with other nucleotide sequences. This modular function facilitates the engineering of RNAs and DNAs that have more complex functions. We sought to create new DNA aptamers that bind cellulose to provide a module for immobilizing DNAs. Cellulose has been used in a variety of applications ranging from coatings and films to pharmaceutical preparations, and therefore DNA aptamers that bind cellulose might enable new applications. We used in vitro selection to isolate aptamers from a pool of random-sequence DNAs and subjected two distinct clones to additional rounds of mutagenesis and selection. One aptamer (CELAPT 14) was chosen for sequence minimization and more detailed biochemical analysis. CELAPT 14 aptamer variants exhibit robust binding both to cellulose powder and paper. Also, an allosteric aptamer construct was engineered that exhibits ATP-mediated cellulose binding during paper chromatography.
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spelling pubmed-20958002007-12-07 In vitro selection and characterization of cellulose-binding DNA aptamers Boese, Benjamin J. Breaker, Ronald R. Nucleic Acids Res Molecular Biology Many nucleic acid enzymes and aptamers have modular architectures that allow them to retain their functions when combined with other nucleotide sequences. This modular function facilitates the engineering of RNAs and DNAs that have more complex functions. We sought to create new DNA aptamers that bind cellulose to provide a module for immobilizing DNAs. Cellulose has been used in a variety of applications ranging from coatings and films to pharmaceutical preparations, and therefore DNA aptamers that bind cellulose might enable new applications. We used in vitro selection to isolate aptamers from a pool of random-sequence DNAs and subjected two distinct clones to additional rounds of mutagenesis and selection. One aptamer (CELAPT 14) was chosen for sequence minimization and more detailed biochemical analysis. CELAPT 14 aptamer variants exhibit robust binding both to cellulose powder and paper. Also, an allosteric aptamer construct was engineered that exhibits ATP-mediated cellulose binding during paper chromatography. Oxford University Press 2007-10 2007-09-18 /pmc/articles/PMC2095800/ /pubmed/17881365 http://dx.doi.org/10.1093/nar/gkm708 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Boese, Benjamin J.
Breaker, Ronald R.
In vitro selection and characterization of cellulose-binding DNA aptamers
title In vitro selection and characterization of cellulose-binding DNA aptamers
title_full In vitro selection and characterization of cellulose-binding DNA aptamers
title_fullStr In vitro selection and characterization of cellulose-binding DNA aptamers
title_full_unstemmed In vitro selection and characterization of cellulose-binding DNA aptamers
title_short In vitro selection and characterization of cellulose-binding DNA aptamers
title_sort in vitro selection and characterization of cellulose-binding dna aptamers
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2095800/
https://www.ncbi.nlm.nih.gov/pubmed/17881365
http://dx.doi.org/10.1093/nar/gkm708
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