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The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores
P2Y(2) receptors, which are equally responsive to ATP and UTP, can trigger intracellular signaling events, such as intracellular calcium mobilization and mitogen-activated protein (MAP) kinase phosphorylation in polymorphonuclear leukocytes (PMN). Moreover, extracellular nucleotides have been shown...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer Netherlands
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2096557/ https://www.ncbi.nlm.nih.gov/pubmed/18404520 http://dx.doi.org/10.1007/s11302-005-0039-8 |
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author | Tuluc, Florin Bredetean, Ovidiu Brailoiu, Eugen Meshki, John Garcia, Analia Dun, Nae J. Kunapuli, Satya P. |
author_facet | Tuluc, Florin Bredetean, Ovidiu Brailoiu, Eugen Meshki, John Garcia, Analia Dun, Nae J. Kunapuli, Satya P. |
author_sort | Tuluc, Florin |
collection | PubMed |
description | P2Y(2) receptors, which are equally responsive to ATP and UTP, can trigger intracellular signaling events, such as intracellular calcium mobilization and mitogen-activated protein (MAP) kinase phosphorylation in polymorphonuclear leukocytes (PMN). Moreover, extracellular nucleotides have been shown to prime chemoattractant-induced superoxide production. The aim of our study was to investigate the mechanism responsible for the priming effect of extracellular nucleotides on reactive oxygen species (ROS) production induced in human neutrophils by two different chemoattractants: formyl-methionyl-leucyl-phenylalanine (fMLP) and interleukin-8 (IL-8). Nucleotide-induced priming of ROS production was concentration- and time-dependent. When UTP was added to neutrophil suspensions prior to chemoattractant, the increase of the response reached the maximum at 1 min of pre-incubation with the nucleotide. UTP potentiated the phosphorylation of p44/42 and p38 MAP kinases induced by chemoattractants, however the P2 receptor-mediated potentiation of ROS production was still detectable in the presence of a SB203580 or U0126, supporting the view that MAP kinases do not play a major role in regulating the nucleotide-induced effect. In the presence of thapsigargin, an inhibitor of the ubiquitous sarco-endoplasmic reticulum Ca(2+)-ATPases in mammalian cells, the effect of fMLP was not affected, but UTP-induced priming was abolished, suggesting that the release of calcium from thapsigargin-sensitive intracellular stores is essential for nucleotide-induced priming in human neutrophils. |
format | Text |
id | pubmed-2096557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-20965572008-02-27 The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores Tuluc, Florin Bredetean, Ovidiu Brailoiu, Eugen Meshki, John Garcia, Analia Dun, Nae J. Kunapuli, Satya P. Purinergic Signal Article P2Y(2) receptors, which are equally responsive to ATP and UTP, can trigger intracellular signaling events, such as intracellular calcium mobilization and mitogen-activated protein (MAP) kinase phosphorylation in polymorphonuclear leukocytes (PMN). Moreover, extracellular nucleotides have been shown to prime chemoattractant-induced superoxide production. The aim of our study was to investigate the mechanism responsible for the priming effect of extracellular nucleotides on reactive oxygen species (ROS) production induced in human neutrophils by two different chemoattractants: formyl-methionyl-leucyl-phenylalanine (fMLP) and interleukin-8 (IL-8). Nucleotide-induced priming of ROS production was concentration- and time-dependent. When UTP was added to neutrophil suspensions prior to chemoattractant, the increase of the response reached the maximum at 1 min of pre-incubation with the nucleotide. UTP potentiated the phosphorylation of p44/42 and p38 MAP kinases induced by chemoattractants, however the P2 receptor-mediated potentiation of ROS production was still detectable in the presence of a SB203580 or U0126, supporting the view that MAP kinases do not play a major role in regulating the nucleotide-induced effect. In the presence of thapsigargin, an inhibitor of the ubiquitous sarco-endoplasmic reticulum Ca(2+)-ATPases in mammalian cells, the effect of fMLP was not affected, but UTP-induced priming was abolished, suggesting that the release of calcium from thapsigargin-sensitive intracellular stores is essential for nucleotide-induced priming in human neutrophils. Springer Netherlands 2005-12-03 2005-12 /pmc/articles/PMC2096557/ /pubmed/18404520 http://dx.doi.org/10.1007/s11302-005-0039-8 Text en © Springer 2005 |
spellingShingle | Article Tuluc, Florin Bredetean, Ovidiu Brailoiu, Eugen Meshki, John Garcia, Analia Dun, Nae J. Kunapuli, Satya P. The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores |
title | The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores |
title_full | The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores |
title_fullStr | The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores |
title_full_unstemmed | The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores |
title_short | The priming effect of extracellular UTP on human neutrophils: Role of calcium released from thapsigargin-sensitive intracellular stores |
title_sort | priming effect of extracellular utp on human neutrophils: role of calcium released from thapsigargin-sensitive intracellular stores |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2096557/ https://www.ncbi.nlm.nih.gov/pubmed/18404520 http://dx.doi.org/10.1007/s11302-005-0039-8 |
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