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Megf10 regulates the progression of the satellite cell myogenic program
We identify here the multiple epidermal growth factor repeat transmembrane protein Megf10 as a quiescent satellite cell marker that is also expressed in skeletal myoblasts but not in differentiated myofibers. Retroviral expression of Megf10 in myoblasts results in enhanced proliferation and inhibite...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099186/ https://www.ncbi.nlm.nih.gov/pubmed/18056409 http://dx.doi.org/10.1083/jcb.200709083 |
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author | Holterman, Chet E. Le Grand, Fabien Kuang, Shihuan Seale, Patrick Rudnicki, Michael A. |
author_facet | Holterman, Chet E. Le Grand, Fabien Kuang, Shihuan Seale, Patrick Rudnicki, Michael A. |
author_sort | Holterman, Chet E. |
collection | PubMed |
description | We identify here the multiple epidermal growth factor repeat transmembrane protein Megf10 as a quiescent satellite cell marker that is also expressed in skeletal myoblasts but not in differentiated myofibers. Retroviral expression of Megf10 in myoblasts results in enhanced proliferation and inhibited differentiation. Infected myoblasts that fail to differentiate undergo cell cycle arrest and can reenter the cell cycle upon serum restimulation. Moreover, experimental modulations of Megf10 alter the expression levels of Pax7 and the myogenic regulatory factors. In contrast, Megf10 silencing in activated satellite cells on individual fibers or in cultured myoblasts results in a dramatic reduction in the cell number, caused by myogenin activation and precocious differentiation as well as a depletion of the self-renewing Pax7(+)/MyoD(−) population. Additionally, Megf10 silencing in MyoD (−/−) myoblasts results in down-regulation of Notch signaling components. We conclude that Megf10 represents a novel transmembrane protein that impinges on Notch signaling to regulate the satellite cell population balance between proliferation and differentiation. |
format | Text |
id | pubmed-2099186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20991862008-06-03 Megf10 regulates the progression of the satellite cell myogenic program Holterman, Chet E. Le Grand, Fabien Kuang, Shihuan Seale, Patrick Rudnicki, Michael A. J Cell Biol Research Articles We identify here the multiple epidermal growth factor repeat transmembrane protein Megf10 as a quiescent satellite cell marker that is also expressed in skeletal myoblasts but not in differentiated myofibers. Retroviral expression of Megf10 in myoblasts results in enhanced proliferation and inhibited differentiation. Infected myoblasts that fail to differentiate undergo cell cycle arrest and can reenter the cell cycle upon serum restimulation. Moreover, experimental modulations of Megf10 alter the expression levels of Pax7 and the myogenic regulatory factors. In contrast, Megf10 silencing in activated satellite cells on individual fibers or in cultured myoblasts results in a dramatic reduction in the cell number, caused by myogenin activation and precocious differentiation as well as a depletion of the self-renewing Pax7(+)/MyoD(−) population. Additionally, Megf10 silencing in MyoD (−/−) myoblasts results in down-regulation of Notch signaling components. We conclude that Megf10 represents a novel transmembrane protein that impinges on Notch signaling to regulate the satellite cell population balance between proliferation and differentiation. The Rockefeller University Press 2007-12-03 /pmc/articles/PMC2099186/ /pubmed/18056409 http://dx.doi.org/10.1083/jcb.200709083 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Holterman, Chet E. Le Grand, Fabien Kuang, Shihuan Seale, Patrick Rudnicki, Michael A. Megf10 regulates the progression of the satellite cell myogenic program |
title | Megf10 regulates the progression of the satellite cell myogenic program |
title_full | Megf10 regulates the progression of the satellite cell myogenic program |
title_fullStr | Megf10 regulates the progression of the satellite cell myogenic program |
title_full_unstemmed | Megf10 regulates the progression of the satellite cell myogenic program |
title_short | Megf10 regulates the progression of the satellite cell myogenic program |
title_sort | megf10 regulates the progression of the satellite cell myogenic program |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099186/ https://www.ncbi.nlm.nih.gov/pubmed/18056409 http://dx.doi.org/10.1083/jcb.200709083 |
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