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In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids

Type II par operons harness polymerization of the dynamically unstable actin-like protein ParM to segregate low-copy plasmids in rod-shaped bacteria. In this study, we use time-lapse fluorescence microscopy to follow plasmid dynamics and ParM assembly in Escherichia coli. Plasmids lacking a par oper...

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Detalles Bibliográficos
Autores principales: Campbell, Christopher S., Mullins, R. Dyche
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099209/
https://www.ncbi.nlm.nih.gov/pubmed/18039937
http://dx.doi.org/10.1083/jcb.200708206
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author Campbell, Christopher S.
Mullins, R. Dyche
author_facet Campbell, Christopher S.
Mullins, R. Dyche
author_sort Campbell, Christopher S.
collection PubMed
description Type II par operons harness polymerization of the dynamically unstable actin-like protein ParM to segregate low-copy plasmids in rod-shaped bacteria. In this study, we use time-lapse fluorescence microscopy to follow plasmid dynamics and ParM assembly in Escherichia coli. Plasmids lacking a par operon undergo confined diffusion with a diffusion constant of 5 × 10(−5) μm(2)/s and a confinement radius of 0.28 μm. Single par-containing plasmids also move diffusively but with a larger diffusion constant (4 × 10(−4) μm(2)/s) and confinement radius (0.42 μm). ParM filaments are dynamically unstable in vivo and form spindles that link pairs of par-containing plasmids and drive them rapidly (3.1 μm/min) toward opposite poles of the cell. After reaching the poles, ParM filaments rapidly and completely depolymerize. After ParM disassembly, segregated plasmids resume diffusive motion, often encountering each other many times and undergoing multiple rounds of ParM-dependent segregation in a single cell cycle. We propose that in addition to driving segregation, the par operon enables plasmids to search space and find sister plasmids more effectively.
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spelling pubmed-20992092008-06-03 In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids Campbell, Christopher S. Mullins, R. Dyche J Cell Biol Research Articles Type II par operons harness polymerization of the dynamically unstable actin-like protein ParM to segregate low-copy plasmids in rod-shaped bacteria. In this study, we use time-lapse fluorescence microscopy to follow plasmid dynamics and ParM assembly in Escherichia coli. Plasmids lacking a par operon undergo confined diffusion with a diffusion constant of 5 × 10(−5) μm(2)/s and a confinement radius of 0.28 μm. Single par-containing plasmids also move diffusively but with a larger diffusion constant (4 × 10(−4) μm(2)/s) and confinement radius (0.42 μm). ParM filaments are dynamically unstable in vivo and form spindles that link pairs of par-containing plasmids and drive them rapidly (3.1 μm/min) toward opposite poles of the cell. After reaching the poles, ParM filaments rapidly and completely depolymerize. After ParM disassembly, segregated plasmids resume diffusive motion, often encountering each other many times and undergoing multiple rounds of ParM-dependent segregation in a single cell cycle. We propose that in addition to driving segregation, the par operon enables plasmids to search space and find sister plasmids more effectively. The Rockefeller University Press 2007-12-03 /pmc/articles/PMC2099209/ /pubmed/18039937 http://dx.doi.org/10.1083/jcb.200708206 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Campbell, Christopher S.
Mullins, R. Dyche
In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids
title In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids
title_full In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids
title_fullStr In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids
title_full_unstemmed In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids
title_short In vivo visualization of type II plasmid segregation: bacterial actin filaments pushing plasmids
title_sort in vivo visualization of type ii plasmid segregation: bacterial actin filaments pushing plasmids
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099209/
https://www.ncbi.nlm.nih.gov/pubmed/18039937
http://dx.doi.org/10.1083/jcb.200708206
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