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Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
BACKGROUND: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. AIM: The present study was assess to investigate the activities of the esse...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099429/ https://www.ncbi.nlm.nih.gov/pubmed/17894898 http://dx.doi.org/10.1186/1476-0711-6-10 |
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author | Saddi, Manuela Sanna, Adriana Cottiglia, Filippo Chisu, Lorenza Casu, Laura Bonsignore, Leonardo De Logu, Alessandro |
author_facet | Saddi, Manuela Sanna, Adriana Cottiglia, Filippo Chisu, Lorenza Casu, Laura Bonsignore, Leonardo De Logu, Alessandro |
author_sort | Saddi, Manuela |
collection | PubMed |
description | BACKGROUND: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. AIM: The present study was assess to investigate the activities of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2 METHODS: The cytotoxicity in Vero cells was evaluated by the MTT reduction method. The IC(50 )values were determined by plaque reduction assay. In order to characterize the mechanism of action, yield reduction assay, inhibition of plaque development assay, attachment assay, penetration assay and post-attachment virus neutralization assay were also performed. RESULTS: The IC(50 )values, determined by plaque reduction assay, were 2.4 and 4.1 μg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC(50 )value of 132 μg/ml, indicating a CC(50)/IC(50 )ratio of 55 for HSV-1 and 32.2 for HSV-2. The antiviral activity of A. arborescens essential oil is principally due to direct virucidal effects. A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No inhibition was observed by attachment assay, penetration assay and post-attachment virus neutralization assay. Furthermore, inhibition of plaque development assay showed that A. arborescens essential oil inhibits the lateral diffusion of both HSV-1 and HSV-2. CONCLUSION: This study demonstrates the antiviral activity of the essential oil in toto obtained from A. arborescens against HSV-1 and HSV-2. The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion. |
format | Text |
id | pubmed-2099429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-20994292007-11-30 Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells Saddi, Manuela Sanna, Adriana Cottiglia, Filippo Chisu, Lorenza Casu, Laura Bonsignore, Leonardo De Logu, Alessandro Ann Clin Microbiol Antimicrob Research BACKGROUND: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. AIM: The present study was assess to investigate the activities of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2 METHODS: The cytotoxicity in Vero cells was evaluated by the MTT reduction method. The IC(50 )values were determined by plaque reduction assay. In order to characterize the mechanism of action, yield reduction assay, inhibition of plaque development assay, attachment assay, penetration assay and post-attachment virus neutralization assay were also performed. RESULTS: The IC(50 )values, determined by plaque reduction assay, were 2.4 and 4.1 μg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC(50 )value of 132 μg/ml, indicating a CC(50)/IC(50 )ratio of 55 for HSV-1 and 32.2 for HSV-2. The antiviral activity of A. arborescens essential oil is principally due to direct virucidal effects. A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No inhibition was observed by attachment assay, penetration assay and post-attachment virus neutralization assay. Furthermore, inhibition of plaque development assay showed that A. arborescens essential oil inhibits the lateral diffusion of both HSV-1 and HSV-2. CONCLUSION: This study demonstrates the antiviral activity of the essential oil in toto obtained from A. arborescens against HSV-1 and HSV-2. The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion. BioMed Central 2007-09-26 /pmc/articles/PMC2099429/ /pubmed/17894898 http://dx.doi.org/10.1186/1476-0711-6-10 Text en Copyright © 2007 Saddi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Saddi, Manuela Sanna, Adriana Cottiglia, Filippo Chisu, Lorenza Casu, Laura Bonsignore, Leonardo De Logu, Alessandro Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells |
title | Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells |
title_full | Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells |
title_fullStr | Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells |
title_full_unstemmed | Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells |
title_short | Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells |
title_sort | antiherpevirus activity of artemisia arborescens essential oil and inhibition of lateral diffusion in vero cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099429/ https://www.ncbi.nlm.nih.gov/pubmed/17894898 http://dx.doi.org/10.1186/1476-0711-6-10 |
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