Cargando…

Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells

BACKGROUND: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. AIM: The present study was assess to investigate the activities of the esse...

Descripción completa

Detalles Bibliográficos
Autores principales: Saddi, Manuela, Sanna, Adriana, Cottiglia, Filippo, Chisu, Lorenza, Casu, Laura, Bonsignore, Leonardo, De Logu, Alessandro
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099429/
https://www.ncbi.nlm.nih.gov/pubmed/17894898
http://dx.doi.org/10.1186/1476-0711-6-10
_version_ 1782138306518056960
author Saddi, Manuela
Sanna, Adriana
Cottiglia, Filippo
Chisu, Lorenza
Casu, Laura
Bonsignore, Leonardo
De Logu, Alessandro
author_facet Saddi, Manuela
Sanna, Adriana
Cottiglia, Filippo
Chisu, Lorenza
Casu, Laura
Bonsignore, Leonardo
De Logu, Alessandro
author_sort Saddi, Manuela
collection PubMed
description BACKGROUND: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. AIM: The present study was assess to investigate the activities of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2 METHODS: The cytotoxicity in Vero cells was evaluated by the MTT reduction method. The IC(50 )values were determined by plaque reduction assay. In order to characterize the mechanism of action, yield reduction assay, inhibition of plaque development assay, attachment assay, penetration assay and post-attachment virus neutralization assay were also performed. RESULTS: The IC(50 )values, determined by plaque reduction assay, were 2.4 and 4.1 μg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC(50 )value of 132 μg/ml, indicating a CC(50)/IC(50 )ratio of 55 for HSV-1 and 32.2 for HSV-2. The antiviral activity of A. arborescens essential oil is principally due to direct virucidal effects. A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No inhibition was observed by attachment assay, penetration assay and post-attachment virus neutralization assay. Furthermore, inhibition of plaque development assay showed that A. arborescens essential oil inhibits the lateral diffusion of both HSV-1 and HSV-2. CONCLUSION: This study demonstrates the antiviral activity of the essential oil in toto obtained from A. arborescens against HSV-1 and HSV-2. The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion.
format Text
id pubmed-2099429
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-20994292007-11-30 Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells Saddi, Manuela Sanna, Adriana Cottiglia, Filippo Chisu, Lorenza Casu, Laura Bonsignore, Leonardo De Logu, Alessandro Ann Clin Microbiol Antimicrob Research BACKGROUND: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. AIM: The present study was assess to investigate the activities of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2 METHODS: The cytotoxicity in Vero cells was evaluated by the MTT reduction method. The IC(50 )values were determined by plaque reduction assay. In order to characterize the mechanism of action, yield reduction assay, inhibition of plaque development assay, attachment assay, penetration assay and post-attachment virus neutralization assay were also performed. RESULTS: The IC(50 )values, determined by plaque reduction assay, were 2.4 and 4.1 μg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC(50 )value of 132 μg/ml, indicating a CC(50)/IC(50 )ratio of 55 for HSV-1 and 32.2 for HSV-2. The antiviral activity of A. arborescens essential oil is principally due to direct virucidal effects. A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No inhibition was observed by attachment assay, penetration assay and post-attachment virus neutralization assay. Furthermore, inhibition of plaque development assay showed that A. arborescens essential oil inhibits the lateral diffusion of both HSV-1 and HSV-2. CONCLUSION: This study demonstrates the antiviral activity of the essential oil in toto obtained from A. arborescens against HSV-1 and HSV-2. The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion. BioMed Central 2007-09-26 /pmc/articles/PMC2099429/ /pubmed/17894898 http://dx.doi.org/10.1186/1476-0711-6-10 Text en Copyright © 2007 Saddi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Saddi, Manuela
Sanna, Adriana
Cottiglia, Filippo
Chisu, Lorenza
Casu, Laura
Bonsignore, Leonardo
De Logu, Alessandro
Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
title Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
title_full Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
title_fullStr Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
title_full_unstemmed Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
title_short Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells
title_sort antiherpevirus activity of artemisia arborescens essential oil and inhibition of lateral diffusion in vero cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2099429/
https://www.ncbi.nlm.nih.gov/pubmed/17894898
http://dx.doi.org/10.1186/1476-0711-6-10
work_keys_str_mv AT saddimanuela antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells
AT sannaadriana antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells
AT cottigliafilippo antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells
AT chisulorenza antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells
AT casulaura antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells
AT bonsignoreleonardo antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells
AT delogualessandro antiherpevirusactivityofartemisiaarborescensessentialoilandinhibitionoflateraldiffusioninverocells