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INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen

Some properties of rat spleen ribonuclease have been studied, and the intracellular distribution of the enzyme and ribonucleic acid have been presented. Spleen ribonuclease exhibits maximal activity at pH 5.8, and although there is some evidence for the presence of an enzyme with an optimum at pH 7....

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Detalles Bibliográficos
Autores principales: Eichel, Herbert J., Roth, Jay S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106032/
https://www.ncbi.nlm.nih.gov/pubmed/13889545
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author Eichel, Herbert J.
Roth, Jay S.
author_facet Eichel, Herbert J.
Roth, Jay S.
author_sort Eichel, Herbert J.
collection PubMed
description Some properties of rat spleen ribonuclease have been studied, and the intracellular distribution of the enzyme and ribonucleic acid have been presented. Spleen ribonuclease exhibits maximal activity at pH 5.8, and although there is some evidence for the presence of an enzyme with an optimum at pH 7.0, it is not conclusive. The enzyme is concentrated primarily in the mitochondrial fraction, but significant quantities occur in the supernatant fluid. The latter contains ribonuclease inhibitor similar to that found in liver. The effects of whole body x-irradiation, magnesium ion, substrate concentration, type of buffer, presence of p-chloromercuriphenylsulfonic acid, deoxycholate, and Triton X-100 on ribonuclease activity are examined.
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spelling pubmed-21060322008-05-01 INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen Eichel, Herbert J. Roth, Jay S. J Cell Biol Article Some properties of rat spleen ribonuclease have been studied, and the intracellular distribution of the enzyme and ribonucleic acid have been presented. Spleen ribonuclease exhibits maximal activity at pH 5.8, and although there is some evidence for the presence of an enzyme with an optimum at pH 7.0, it is not conclusive. The enzyme is concentrated primarily in the mitochondrial fraction, but significant quantities occur in the supernatant fluid. The latter contains ribonuclease inhibitor similar to that found in liver. The effects of whole body x-irradiation, magnesium ion, substrate concentration, type of buffer, presence of p-chloromercuriphenylsulfonic acid, deoxycholate, and Triton X-100 on ribonuclease activity are examined. The Rockefeller University Press 1962-02-01 /pmc/articles/PMC2106032/ /pubmed/13889545 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Eichel, Herbert J.
Roth, Jay S.
INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen
title INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen
title_full INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen
title_fullStr INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen
title_full_unstemmed INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen
title_short INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN : II. Some Properties and the Distribution of Ribonuclease in Rat Spleen
title_sort intracellular localization of enzymes in spleen : ii. some properties and the distribution of ribonuclease in rat spleen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106032/
https://www.ncbi.nlm.nih.gov/pubmed/13889545
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