FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE

This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The...

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Detalles Bibliográficos
Autores principales: Palay, Sanford L., McGee-Russell, S. M., Gordon, Spencer, Grillo, Mary A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106034/
https://www.ncbi.nlm.nih.gov/pubmed/14483299
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author Palay, Sanford L.
McGee-Russell, S. M.
Gordon, Spencer
Grillo, Mary A.
author_facet Palay, Sanford L.
McGee-Russell, S. M.
Gordon, Spencer
Grillo, Mary A.
author_sort Palay, Sanford L.
collection PubMed
description This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.
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spelling pubmed-21060342008-05-01 FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE Palay, Sanford L. McGee-Russell, S. M. Gordon, Spencer Grillo, Mary A. J Cell Biol Article This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore. The Rockefeller University Press 1962-02-01 /pmc/articles/PMC2106034/ /pubmed/14483299 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Palay, Sanford L.
McGee-Russell, S. M.
Gordon, Spencer
Grillo, Mary A.
FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
title FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
title_full FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
title_fullStr FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
title_full_unstemmed FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
title_short FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE
title_sort fixation of neural tissues for electron microscopy by perfusion with solutions of osmium tetroxide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106034/
https://www.ncbi.nlm.nih.gov/pubmed/14483299
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AT gordonspencer fixationofneuraltissuesforelectronmicroscopybyperfusionwithsolutionsofosmiumtetroxide
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