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STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro

In vitro studies of the transport of colloidal particles by the cornea were carried out on intact corneas of adult rabbits in a chamber described by Donn, Maurice, and Mills (2) in which the epithelial or the endothelial surface of the cornea was exposed to thorium dioxide or saccharated iron oxide...

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Detalles Bibliográficos
Autores principales: Kaye, Gordon I., Pappas, George D., Donn, Anthony, Mallett, Nancy
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106050/
https://www.ncbi.nlm.nih.gov/pubmed/14454674
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author Kaye, Gordon I.
Pappas, George D.
Donn, Anthony
Mallett, Nancy
author_facet Kaye, Gordon I.
Pappas, George D.
Donn, Anthony
Mallett, Nancy
author_sort Kaye, Gordon I.
collection PubMed
description In vitro studies of the transport of colloidal particles by the cornea were carried out on intact corneas of adult rabbits in a chamber described by Donn, Maurice, and Mills (2) in which the epithelial or the endothelial surface of the cornea was exposed to thorium dioxide or saccharated iron oxide under various conditions. These studies confirmed the results of previous work in vivo and allowed modification of the experimental conditions. Particles are pinocytosed at the apical surface of the corneal endothelium and carried around the terminal bar in membrane-bounded vesicles. Basal to the terminal bar these vesicles fuse with the lateral cell margin and their contents are released into the intercellular space, in which they appear to be carried by a one-way flow down to Descemet's membrane and the corneal stroma. Indications that the endothelial transport is an active process are presented by the different pathways of transport into or out of the corneal stroma, as well as by the approximately 70 per cent reduction in transport activity at low temperatures.
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spelling pubmed-21060502008-05-01 STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro Kaye, Gordon I. Pappas, George D. Donn, Anthony Mallett, Nancy J Cell Biol Article In vitro studies of the transport of colloidal particles by the cornea were carried out on intact corneas of adult rabbits in a chamber described by Donn, Maurice, and Mills (2) in which the epithelial or the endothelial surface of the cornea was exposed to thorium dioxide or saccharated iron oxide under various conditions. These studies confirmed the results of previous work in vivo and allowed modification of the experimental conditions. Particles are pinocytosed at the apical surface of the corneal endothelium and carried around the terminal bar in membrane-bounded vesicles. Basal to the terminal bar these vesicles fuse with the lateral cell margin and their contents are released into the intercellular space, in which they appear to be carried by a one-way flow down to Descemet's membrane and the corneal stroma. Indications that the endothelial transport is an active process are presented by the different pathways of transport into or out of the corneal stroma, as well as by the approximately 70 per cent reduction in transport activity at low temperatures. The Rockefeller University Press 1962-03-01 /pmc/articles/PMC2106050/ /pubmed/14454674 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kaye, Gordon I.
Pappas, George D.
Donn, Anthony
Mallett, Nancy
STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
title STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
title_full STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
title_fullStr STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
title_full_unstemmed STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
title_short STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
title_sort studies on the cornea : ii. the uptake and transport of colloidal particles by the living rabbit cornea in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106050/
https://www.ncbi.nlm.nih.gov/pubmed/14454674
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