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STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro
In vitro studies of the transport of colloidal particles by the cornea were carried out on intact corneas of adult rabbits in a chamber described by Donn, Maurice, and Mills (2) in which the epithelial or the endothelial surface of the cornea was exposed to thorium dioxide or saccharated iron oxide...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1962
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106050/ https://www.ncbi.nlm.nih.gov/pubmed/14454674 |
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author | Kaye, Gordon I. Pappas, George D. Donn, Anthony Mallett, Nancy |
author_facet | Kaye, Gordon I. Pappas, George D. Donn, Anthony Mallett, Nancy |
author_sort | Kaye, Gordon I. |
collection | PubMed |
description | In vitro studies of the transport of colloidal particles by the cornea were carried out on intact corneas of adult rabbits in a chamber described by Donn, Maurice, and Mills (2) in which the epithelial or the endothelial surface of the cornea was exposed to thorium dioxide or saccharated iron oxide under various conditions. These studies confirmed the results of previous work in vivo and allowed modification of the experimental conditions. Particles are pinocytosed at the apical surface of the corneal endothelium and carried around the terminal bar in membrane-bounded vesicles. Basal to the terminal bar these vesicles fuse with the lateral cell margin and their contents are released into the intercellular space, in which they appear to be carried by a one-way flow down to Descemet's membrane and the corneal stroma. Indications that the endothelial transport is an active process are presented by the different pathways of transport into or out of the corneal stroma, as well as by the approximately 70 per cent reduction in transport activity at low temperatures. |
format | Text |
id | pubmed-2106050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1962 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21060502008-05-01 STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro Kaye, Gordon I. Pappas, George D. Donn, Anthony Mallett, Nancy J Cell Biol Article In vitro studies of the transport of colloidal particles by the cornea were carried out on intact corneas of adult rabbits in a chamber described by Donn, Maurice, and Mills (2) in which the epithelial or the endothelial surface of the cornea was exposed to thorium dioxide or saccharated iron oxide under various conditions. These studies confirmed the results of previous work in vivo and allowed modification of the experimental conditions. Particles are pinocytosed at the apical surface of the corneal endothelium and carried around the terminal bar in membrane-bounded vesicles. Basal to the terminal bar these vesicles fuse with the lateral cell margin and their contents are released into the intercellular space, in which they appear to be carried by a one-way flow down to Descemet's membrane and the corneal stroma. Indications that the endothelial transport is an active process are presented by the different pathways of transport into or out of the corneal stroma, as well as by the approximately 70 per cent reduction in transport activity at low temperatures. The Rockefeller University Press 1962-03-01 /pmc/articles/PMC2106050/ /pubmed/14454674 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kaye, Gordon I. Pappas, George D. Donn, Anthony Mallett, Nancy STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro |
title | STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro |
title_full | STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro |
title_fullStr | STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro |
title_full_unstemmed | STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro |
title_short | STUDIES ON THE CORNEA : II. The Uptake and Transport of Colloidal Particles by the Living Rabbit Cornea in Vitro |
title_sort | studies on the cornea : ii. the uptake and transport of colloidal particles by the living rabbit cornea in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106050/ https://www.ncbi.nlm.nih.gov/pubmed/14454674 |
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