A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY

A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low...

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Detalles Bibliográficos
Autores principales: Freeman, James A., Spurlock, Ben O.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106084/
https://www.ncbi.nlm.nih.gov/pubmed/13894888
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author Freeman, James A.
Spurlock, Ben O.
author_facet Freeman, James A.
Spurlock, Ben O.
author_sort Freeman, James A.
collection PubMed
description A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low viscosity, and, most important, ease of sectioning on a Porter-Blum microtome. In contrast to Epon-812-embedded tissues, Maraglas-Cardolite-embedded tissues can be sectioned in large dimensions with ease and consistent results without "chatter." No background granularity is detectable with high magnification study of Maraglas-Cardolite-embedded tissues. This epoxy is readily stained with lead hydroxide and is relatively stable in the electron beam.
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spelling pubmed-21060842008-05-01 A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY Freeman, James A. Spurlock, Ben O. J Cell Biol Article A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low viscosity, and, most important, ease of sectioning on a Porter-Blum microtome. In contrast to Epon-812-embedded tissues, Maraglas-Cardolite-embedded tissues can be sectioned in large dimensions with ease and consistent results without "chatter." No background granularity is detectable with high magnification study of Maraglas-Cardolite-embedded tissues. This epoxy is readily stained with lead hydroxide and is relatively stable in the electron beam. The Rockefeller University Press 1962-06-01 /pmc/articles/PMC2106084/ /pubmed/13894888 Text en Copyright © 1962 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Freeman, James A.
Spurlock, Ben O.
A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
title A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
title_full A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
title_fullStr A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
title_full_unstemmed A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
title_short A NEW EPOXY EMBEDMENT FOR ELECTRON MICROSCOPY
title_sort new epoxy embedment for electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106084/
https://www.ncbi.nlm.nih.gov/pubmed/13894888
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AT spurlockbeno anewepoxyembedmentforelectronmicroscopy
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AT spurlockbeno newepoxyembedmentforelectronmicroscopy

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