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ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT

The ultrastructure of the uterine epithelium has been studied in estrous, ovariectomized, pregnant, and pseudopregnant rabbits. Tissue for light microscopy was fixed in Bouin's solution and stained with hematoxylin and eosin, by the periodic acid-Schiff (PAS) method, and with methylene blue. Ti...

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Autor principal: Larsen, Jørgen Falck
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106085/
https://www.ncbi.nlm.nih.gov/pubmed/14462496
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author Larsen, Jørgen Falck
author_facet Larsen, Jørgen Falck
author_sort Larsen, Jørgen Falck
collection PubMed
description The ultrastructure of the uterine epithelium has been studied in estrous, ovariectomized, pregnant, and pseudopregnant rabbits. Tissue for light microscopy was fixed in Bouin's solution and stained with hematoxylin and eosin, by the periodic acid-Schiff (PAS) method, and with methylene blue. Tissue for electron microscopy was fixed in 1 per cent osmium tetroxide in White's saline and embedded in Araldite. The uterine epithelium in estrus is comprised of ciliated and non-ciliated cells. After ovariectomy the epithelium becomes reduced in height and PAS-positive material disappears. Multinucleated cells are formed in the epithelium in pregnancy, pseudopregnancy, and in the non-pregnant horn in unilateral pregnancy. They degenerate during the 3rd week of pseudopregnancy and during the 4th week of pregnancy in the non-pregnant horn. The formation of multinucleated cells is believed to be under hormonal control. The uterine epithelium in contact with the blastocyst changes into a "symplasma," presumably under the influence of a local (chemical?) effect produced by the blastocyst. This change is not seen in pseudopregnancy nor in the non-pregnant horn in unilateral pregnancy. A complex infolding of the basal cell membrane of the epithelium accompanies the "symplasmic" change. The remaining uterine epithelium in pregnancy shows a well developed ergastoplasm suggesting a production of secretion materials, some of which may be available for absorption by the fetus through the yolk sac or paraplacental chorion.
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spelling pubmed-21060852008-05-01 ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT Larsen, Jørgen Falck J Cell Biol Article The ultrastructure of the uterine epithelium has been studied in estrous, ovariectomized, pregnant, and pseudopregnant rabbits. Tissue for light microscopy was fixed in Bouin's solution and stained with hematoxylin and eosin, by the periodic acid-Schiff (PAS) method, and with methylene blue. Tissue for electron microscopy was fixed in 1 per cent osmium tetroxide in White's saline and embedded in Araldite. The uterine epithelium in estrus is comprised of ciliated and non-ciliated cells. After ovariectomy the epithelium becomes reduced in height and PAS-positive material disappears. Multinucleated cells are formed in the epithelium in pregnancy, pseudopregnancy, and in the non-pregnant horn in unilateral pregnancy. They degenerate during the 3rd week of pseudopregnancy and during the 4th week of pregnancy in the non-pregnant horn. The formation of multinucleated cells is believed to be under hormonal control. The uterine epithelium in contact with the blastocyst changes into a "symplasma," presumably under the influence of a local (chemical?) effect produced by the blastocyst. This change is not seen in pseudopregnancy nor in the non-pregnant horn in unilateral pregnancy. A complex infolding of the basal cell membrane of the epithelium accompanies the "symplasmic" change. The remaining uterine epithelium in pregnancy shows a well developed ergastoplasm suggesting a production of secretion materials, some of which may be available for absorption by the fetus through the yolk sac or paraplacental chorion. The Rockefeller University Press 1962-07-01 /pmc/articles/PMC2106085/ /pubmed/14462496 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Larsen, Jørgen Falck
ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT
title ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT
title_full ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT
title_fullStr ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT
title_full_unstemmed ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT
title_short ELECTRON MICROSCOPY OF THE UTERINE EPITHELIUM IN THE RABBIT
title_sort electron microscopy of the uterine epithelium in the rabbit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106085/
https://www.ncbi.nlm.nih.gov/pubmed/14462496
work_keys_str_mv AT larsenjørgenfalck electronmicroscopyoftheuterineepitheliumintherabbit