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THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE
Sixteen pregnant female mice were operated on and H(3)-thymidine was injected into the amniotic cavities of the uterus. The injection was given between the 6th and 14th days of fetal life. Eighty-eight fetuses received thymidine in this way. Another series of 16 pregnant females was injected intrape...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1962
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106116/ https://www.ncbi.nlm.nih.gov/pubmed/13930669 |
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author | Lima-de-Faria, A. Borum, Kirstine |
author_facet | Lima-de-Faria, A. Borum, Kirstine |
author_sort | Lima-de-Faria, A. |
collection | PubMed |
description | Sixteen pregnant female mice were operated on and H(3)-thymidine was injected into the amniotic cavities of the uterus. The injection was given between the 6th and 14th days of fetal life. Eighty-eight fetuses received thymidine in this way. Another series of 16 pregnant females was injected intraperitoneally with H(3)-thymidine between the 5th and 14th days of pregnancy. Two of these females were killed 16 days after the observation of the vaginal plug. The remaining 30 females were allowed to give birth to their progeny. The progeny was killed at birth and the ovaries of the newborn females fixed at once. Labeled oocytes at late pachytene and early diplotene were clearly seen in individuals that received the isotope between the 10th and 12th to 13th days of fetal life, but the period of DNA synthesis preceding meiosis is at the 12th to 13th days of fetal life. Since meiosis is recognized by the 14th day, only the oocyte labeling originating from mothers injected at the 12th and 13th days may be considered as representing the DNA synthesis of the premeiotic replication. |
format | Text |
id | pubmed-2106116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1962 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21061162008-05-01 THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE Lima-de-Faria, A. Borum, Kirstine J Cell Biol Article Sixteen pregnant female mice were operated on and H(3)-thymidine was injected into the amniotic cavities of the uterus. The injection was given between the 6th and 14th days of fetal life. Eighty-eight fetuses received thymidine in this way. Another series of 16 pregnant females was injected intraperitoneally with H(3)-thymidine between the 5th and 14th days of pregnancy. Two of these females were killed 16 days after the observation of the vaginal plug. The remaining 30 females were allowed to give birth to their progeny. The progeny was killed at birth and the ovaries of the newborn females fixed at once. Labeled oocytes at late pachytene and early diplotene were clearly seen in individuals that received the isotope between the 10th and 12th to 13th days of fetal life, but the period of DNA synthesis preceding meiosis is at the 12th to 13th days of fetal life. Since meiosis is recognized by the 14th day, only the oocyte labeling originating from mothers injected at the 12th and 13th days may be considered as representing the DNA synthesis of the premeiotic replication. The Rockefeller University Press 1962-09-01 /pmc/articles/PMC2106116/ /pubmed/13930669 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Lima-de-Faria, A. Borum, Kirstine THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE |
title | THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE |
title_full | THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE |
title_fullStr | THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE |
title_full_unstemmed | THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE |
title_short | THE PERIOD OF DNA SYNTHESIS PRIOR TO MEIOSIS IN THE MOUSE |
title_sort | period of dna synthesis prior to meiosis in the mouse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106116/ https://www.ncbi.nlm.nih.gov/pubmed/13930669 |
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