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METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER

Suspensions of isolated cells were prepared from mouse livers that had been perfused via the portal vein with a buffered medium containing sucrose. The demonstration of metabolic activities in these cells was found to be critically dependent on the composition of the suspending medium. The cells sho...

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Detalles Bibliográficos
Autor principal: Berry, M. N.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106128/
https://www.ncbi.nlm.nih.gov/pubmed/19866607
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author Berry, M. N.
author_facet Berry, M. N.
author_sort Berry, M. N.
collection PubMed
description Suspensions of isolated cells were prepared from mouse livers that had been perfused via the portal vein with a buffered medium containing sucrose. The demonstration of metabolic activities in these cells was found to be critically dependent on the composition of the suspending medium. The cells showed considerable metabolic activity in a simple medium containing 0.06 to 0.20 M sucrose, but did not respire in 0.30 M sucrose medium. Endogenous respiration was greatest when the sucrose concentration of the medium was 0.10 M or lower and was associated with the formation of acetoacetate. The cells oxidized citric acid cycle intermediates, glutamate, lactate, pyruvate, β-hydroxybutyrate, α-glycerophosphate, and fatty acids and synthesized urea from ammonium chloride, but carbohydrate substrates did not stimulate oxygen uptake. Cells incubated in Krebs' phosphate-saline did not respire. The lack of respiration in this medium is thought to be related to increased permeability of the cell membrane with penetration of calcium ions and orthophosphate into the cells causing mitochondrial swelling and destruction. Further evidence for the loss of cellular permeability barriers is provided by the demonstration of leakage of certain soluble enzymes into the preparative media.
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spelling pubmed-21061282008-05-01 METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER Berry, M. N. J Cell Biol Article Suspensions of isolated cells were prepared from mouse livers that had been perfused via the portal vein with a buffered medium containing sucrose. The demonstration of metabolic activities in these cells was found to be critically dependent on the composition of the suspending medium. The cells showed considerable metabolic activity in a simple medium containing 0.06 to 0.20 M sucrose, but did not respire in 0.30 M sucrose medium. Endogenous respiration was greatest when the sucrose concentration of the medium was 0.10 M or lower and was associated with the formation of acetoacetate. The cells oxidized citric acid cycle intermediates, glutamate, lactate, pyruvate, β-hydroxybutyrate, α-glycerophosphate, and fatty acids and synthesized urea from ammonium chloride, but carbohydrate substrates did not stimulate oxygen uptake. Cells incubated in Krebs' phosphate-saline did not respire. The lack of respiration in this medium is thought to be related to increased permeability of the cell membrane with penetration of calcium ions and orthophosphate into the cells causing mitochondrial swelling and destruction. Further evidence for the loss of cellular permeability barriers is provided by the demonstration of leakage of certain soluble enzymes into the preparative media. The Rockefeller University Press 1962-10-01 /pmc/articles/PMC2106128/ /pubmed/19866607 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Berry, M. N.
METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER
title METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER
title_full METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER
title_fullStr METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER
title_full_unstemmed METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER
title_short METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER
title_sort metabolic properties of cells isolated from adult mouse liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106128/
https://www.ncbi.nlm.nih.gov/pubmed/19866607
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