THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit

The fine structure of the mitotic apparatus isolated from the sea urchin egg has been investigated. The isolation was accomplished by lysis of metaphase eggs in a 1 M solution of hexanediol, buffered at pH 6. The fine structure of the isolated apparatus was studied after fixation with osmium tetroxi...

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Detalles Bibliográficos
Autor principal: Kane, R. E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106157/
https://www.ncbi.nlm.nih.gov/pubmed/13962188
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author Kane, R. E.
author_facet Kane, R. E.
author_sort Kane, R. E.
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description The fine structure of the mitotic apparatus isolated from the sea urchin egg has been investigated. The isolation was accomplished by lysis of metaphase eggs in a 1 M solution of hexanediol, buffered at pH 6. The fine structure of the isolated apparatus was studied after fixation with osmium tetroxide directly in the isolation medium. The spindle is composed of fine fibrils, approximately 20 mµ in diameter, which appear tubular. Similar fibrils, radially oriented, are found in the aster. If the isolated mitotic apparatus is exposed to water at pH 6 before fixation, the structure is considerably modified. The most pronounced effects are an increase in the number of large membrane-bounded vesicles and in the amount of free granular material present. The conditions necessary for the fixation of the mitotic apparatus in dividing cells are discussed in the light of these observations on the isolated unit.
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spelling pubmed-21061572008-05-01 THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit Kane, R. E. J Cell Biol Article The fine structure of the mitotic apparatus isolated from the sea urchin egg has been investigated. The isolation was accomplished by lysis of metaphase eggs in a 1 M solution of hexanediol, buffered at pH 6. The fine structure of the isolated apparatus was studied after fixation with osmium tetroxide directly in the isolation medium. The spindle is composed of fine fibrils, approximately 20 mµ in diameter, which appear tubular. Similar fibrils, radially oriented, are found in the aster. If the isolated mitotic apparatus is exposed to water at pH 6 before fixation, the structure is considerably modified. The most pronounced effects are an increase in the number of large membrane-bounded vesicles and in the amount of free granular material present. The conditions necessary for the fixation of the mitotic apparatus in dividing cells are discussed in the light of these observations on the isolated unit. The Rockefeller University Press 1962-11-01 /pmc/articles/PMC2106157/ /pubmed/13962188 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kane, R. E.
THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit
title THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit
title_full THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit
title_fullStr THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit
title_full_unstemmed THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit
title_short THE MITOTIC APPARATUS : Fine Structure of the Isolated Unit
title_sort mitotic apparatus : fine structure of the isolated unit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106157/
https://www.ncbi.nlm.nih.gov/pubmed/13962188
work_keys_str_mv AT kanere themitoticapparatusfinestructureoftheisolatedunit
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