CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements

Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae cont...

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Autores principales: Rabinovitch, M., Plaut, W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106167/
https://www.ncbi.nlm.nih.gov/pubmed/13972869
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author Rabinovitch, M.
Plaut, W.
author_facet Rabinovitch, M.
Plaut, W.
author_sort Rabinovitch, M.
collection PubMed
description Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.
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spelling pubmed-21061672008-05-01 CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements Rabinovitch, M. Plaut, W. J Cell Biol Article Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed. The Rockefeller University Press 1962-12-01 /pmc/articles/PMC2106167/ /pubmed/13972869 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rabinovitch, M.
Plaut, W.
CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
title CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
title_full CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
title_fullStr CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
title_full_unstemmed CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
title_short CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : II. On the Behavior and Possible Nature of the DNA-Containing Elements
title_sort cytoplasmic dna synthesis in amoeba proteus : ii. on the behavior and possible nature of the dna-containing elements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106167/
https://www.ncbi.nlm.nih.gov/pubmed/13972869
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