THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN

Nuclei can be isolated from the eggs and embryos of the sea urchin by a four step method: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest o...

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Detalles Bibliográficos
Autor principal: Hinegardner, Ralph T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1962
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106168/
https://www.ncbi.nlm.nih.gov/pubmed/13954643
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author Hinegardner, Ralph T.
author_facet Hinegardner, Ralph T.
author_sort Hinegardner, Ralph T.
collection PubMed
description Nuclei can be isolated from the eggs and embryos of the sea urchin by a four step method: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest of the homogenate by centrifugation on a layered sucrose gradient. Contamination of the isolated nuclei is less than 1 per cent. The yield varies between 20 per cent and 80 per cent depending on the developmental stage used.
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spelling pubmed-21061682008-05-01 THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN Hinegardner, Ralph T. J Cell Biol Article Nuclei can be isolated from the eggs and embryos of the sea urchin by a four step method: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest of the homogenate by centrifugation on a layered sucrose gradient. Contamination of the isolated nuclei is less than 1 per cent. The yield varies between 20 per cent and 80 per cent depending on the developmental stage used. The Rockefeller University Press 1962-12-01 /pmc/articles/PMC2106168/ /pubmed/13954643 Text en Copyright © Copyright, 1962, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hinegardner, Ralph T.
THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
title THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
title_full THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
title_fullStr THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
title_full_unstemmed THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
title_short THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
title_sort isolation of nuclei from eggs and embryos of the sea urchin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106168/
https://www.ncbi.nlm.nih.gov/pubmed/13954643
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