ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei

An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal s...

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Detalles Bibliográficos
Autores principales: Koehler, J. K., Mühlethaler, K., Freywyssling, A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106184/
https://www.ncbi.nlm.nih.gov/pubmed/19866619
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author Koehler, J. K.
Mühlethaler, K.
Freywyssling, A.
author_facet Koehler, J. K.
Mühlethaler, K.
Freywyssling, A.
author_sort Koehler, J. K.
collection PubMed
description An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electron microscopically by this technique and were found to display nuclear labeling with impressive clarity. The new procedure utilized here yields objects in which the finest cell structures are easily resolvable without recourse to gelatin digestion techniques.
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spelling pubmed-21061842008-05-01 ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei Koehler, J. K. Mühlethaler, K. Freywyssling, A. J Cell Biol Article An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electron microscopically by this technique and were found to display nuclear labeling with impressive clarity. The new procedure utilized here yields objects in which the finest cell structures are easily resolvable without recourse to gelatin digestion techniques. The Rockefeller University Press 1963-01-01 /pmc/articles/PMC2106184/ /pubmed/19866619 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Koehler, J. K.
Mühlethaler, K.
Freywyssling, A.
ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei
title ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei
title_full ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei
title_fullStr ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei
title_full_unstemmed ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei
title_short ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H(3)-Thymidine-Labeled Maize Nuclei
title_sort electron microscopic autoradiography : an improved technique for producing thin films and its application to h(3)-thymidine-labeled maize nuclei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106184/
https://www.ncbi.nlm.nih.gov/pubmed/19866619
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AT freywysslinga electronmicroscopicautoradiographyanimprovedtechniqueforproducingthinfilmsanditsapplicationtoh3thymidinelabeledmaizenuclei

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