SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA

A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side o...

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Detalles Bibliográficos
Autor principal: Stoeckenius, Walther
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106204/
https://www.ncbi.nlm.nih.gov/pubmed/13984235
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author Stoeckenius, Walther
author_facet Stoeckenius, Walther
author_sort Stoeckenius, Walther
collection PubMed
description A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side of the mitochondrial envelope which faces the matrix. It is attached to the underlying membrane by a narrow stalk 40 to 50 A long. The particle appears to be rather labile and it is destroyed or at least rendered unrecognizable by all conventional fixatives used in electron microscopy, especially OsO(4).
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spelling pubmed-21062042008-05-01 SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA Stoeckenius, Walther J Cell Biol Article A particulate component of mitochondria is described which is observed in negatively stained preparations of unfixed whole mitochondria or their fragments. It consists of a roughly spherical particle, approximately 85 A in diameter, found on the surface of the cristae and probably also on the side of the mitochondrial envelope which faces the matrix. It is attached to the underlying membrane by a narrow stalk 40 to 50 A long. The particle appears to be rather labile and it is destroyed or at least rendered unrecognizable by all conventional fixatives used in electron microscopy, especially OsO(4). The Rockefeller University Press 1963-05-01 /pmc/articles/PMC2106204/ /pubmed/13984235 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Stoeckenius, Walther
SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
title SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
title_full SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
title_fullStr SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
title_full_unstemmed SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
title_short SOME OBSERVATIONS ON NEGATIVELY STAINED MITOCHONDRIA
title_sort some observations on negatively stained mitochondria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106204/
https://www.ncbi.nlm.nih.gov/pubmed/13984235
work_keys_str_mv AT stoeckeniuswalther someobservationsonnegativelystainedmitochondria

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