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AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS

Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus. The infectious cycle was studied with the electron microscope by means of thin sections of cells fixed from 3 hours up to 11 days after infection. The frequency of virus formatio...

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Autores principales: Granboulan, Nicole, Tournier, P., Wicker, R., Bernhard, W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106205/
https://www.ncbi.nlm.nih.gov/pubmed/13963379
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author Granboulan, Nicole
Tournier, P.
Wicker, R.
Bernhard, W.
author_facet Granboulan, Nicole
Tournier, P.
Wicker, R.
Bernhard, W.
author_sort Granboulan, Nicole
collection PubMed
description Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus. The infectious cycle was studied with the electron microscope by means of thin sections of cells fixed from 3 hours up to 11 days after infection. The frequency of virus formation and various nuclear and cytoplasmic lesions in relation to the infection are described. The virus particles appear in the nucleus in close contact with the chromatin. In a small number of cells they have been observed as early as 10 to 12 hours after infection, but most often they appear 24 to 48 hours afterward. Their mean diameter is 33 mµ. They have no membrane and are frequently arranged as crystal-like structures. In addition to the appearance of virus, one observes various lesions in the nucleoplasm and particularly in the nucleolus, which shows an early hypertrophy and produces unusual, dense condensations in contact with the nucleolonema. The importance of these nucleolar lesions and the relationship between the SV40 virus and the polyoma, common wart, and Shope papilloma viruses are discussed.
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spelling pubmed-21062052008-05-01 AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS Granboulan, Nicole Tournier, P. Wicker, R. Bernhard, W. J Cell Biol Article Kidney cells, predominantly from Cercopithecus monkeys but also from baboons, were infected in vitro with the SV40 virus. The infectious cycle was studied with the electron microscope by means of thin sections of cells fixed from 3 hours up to 11 days after infection. The frequency of virus formation and various nuclear and cytoplasmic lesions in relation to the infection are described. The virus particles appear in the nucleus in close contact with the chromatin. In a small number of cells they have been observed as early as 10 to 12 hours after infection, but most often they appear 24 to 48 hours afterward. Their mean diameter is 33 mµ. They have no membrane and are frequently arranged as crystal-like structures. In addition to the appearance of virus, one observes various lesions in the nucleoplasm and particularly in the nucleolus, which shows an early hypertrophy and produces unusual, dense condensations in contact with the nucleolonema. The importance of these nucleolar lesions and the relationship between the SV40 virus and the polyoma, common wart, and Shope papilloma viruses are discussed. The Rockefeller University Press 1963-05-01 /pmc/articles/PMC2106205/ /pubmed/13963379 Text en Copyright © Copyright, 1963, by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Granboulan, Nicole
Tournier, P.
Wicker, R.
Bernhard, W.
AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS
title AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS
title_full AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS
title_fullStr AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS
title_full_unstemmed AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS
title_short AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF SV40 VIRUS
title_sort electron microscope study of the development of sv40 virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106205/
https://www.ncbi.nlm.nih.gov/pubmed/13963379
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