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STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES
Living excised roots of pea were centrifuged at 20,000 g for 24 hours, and the behavior of organelles was followed by electron microscopy at various intervals after centrifugation. With these forces, organelles are not perceptibly or irreversibly damaged, nor is the viability of the whole root destr...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1963
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106303/ https://www.ncbi.nlm.nih.gov/pubmed/14079500 |
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author | Bouck, G. Benjamin |
author_facet | Bouck, G. Benjamin |
author_sort | Bouck, G. Benjamin |
collection | PubMed |
description | Living excised roots of pea were centrifuged at 20,000 g for 24 hours, and the behavior of organelles was followed by electron microscopy at various intervals after centrifugation. With these forces, organelles are not perceptibly or irreversibly damaged, nor is the viability of the whole root destroyed. Organelles stratify generally in the order of lipid (centripetal pole), vacuoles, smooth endoplasmic reticulum and dictyosomes, proplastids (without starch), mitochondria, rough endoplasmic reticulum, proplastids with starch. The nucleus distends from the vacuolar region to the extreme centrifugal pole of the cell, while the chromatin and nucleolus seek the centrifugal pole of the nucleus. During the redistribution of organelles the rough endoplasmic reticulum is among the first to reorient, and possible explanations for this are discussed. Mitochondria can be stretched elastically many times their original length, but proplastids seem fairly rigid. Small vacuoles, forced together during centrifugation, apparently may fuse to form a large unit. Lipid droplets, on the other hand, tend to remain separate. Dictyosomes and smooth endoplasmic reticulum layer in the same region of the centrifuged cell, indicating a density similarity between these two organelles. |
format | Text |
id | pubmed-2106303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21063032008-05-01 STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES Bouck, G. Benjamin J Cell Biol Article Living excised roots of pea were centrifuged at 20,000 g for 24 hours, and the behavior of organelles was followed by electron microscopy at various intervals after centrifugation. With these forces, organelles are not perceptibly or irreversibly damaged, nor is the viability of the whole root destroyed. Organelles stratify generally in the order of lipid (centripetal pole), vacuoles, smooth endoplasmic reticulum and dictyosomes, proplastids (without starch), mitochondria, rough endoplasmic reticulum, proplastids with starch. The nucleus distends from the vacuolar region to the extreme centrifugal pole of the cell, while the chromatin and nucleolus seek the centrifugal pole of the nucleus. During the redistribution of organelles the rough endoplasmic reticulum is among the first to reorient, and possible explanations for this are discussed. Mitochondria can be stretched elastically many times their original length, but proplastids seem fairly rigid. Small vacuoles, forced together during centrifugation, apparently may fuse to form a large unit. Lipid droplets, on the other hand, tend to remain separate. Dictyosomes and smooth endoplasmic reticulum layer in the same region of the centrifuged cell, indicating a density similarity between these two organelles. The Rockefeller University Press 1963-08-01 /pmc/articles/PMC2106303/ /pubmed/14079500 Text en Copyright © 1963 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Bouck, G. Benjamin STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES |
title | STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES |
title_full | STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES |
title_fullStr | STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES |
title_full_unstemmed | STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES |
title_short | STRATIFICATION AND SUBSEQUENT BEHAVIOR OF PLANT CELL ORGANELLES |
title_sort | stratification and subsequent behavior of plant cell organelles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106303/ https://www.ncbi.nlm.nih.gov/pubmed/14079500 |
work_keys_str_mv | AT bouckgbenjamin stratificationandsubsequentbehaviorofplantcellorganelles |