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STUDIES ON ISOLATED NUCLEI : I. Isolation and Chemical Characterization of a Nuclear Fraction from Guinea Pig Liver
This article describes a method for the isolation of nuclei from guinea pig liver. It involves the homogenization of the tissue in 0.88 M sucrose-1.5 mM CaCl(2) followed by centrifugation in a discontinuous density gradient in which the upper phase is the homogenate and the lower phase is 2.2 M sucr...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1963
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106307/ https://www.ncbi.nlm.nih.gov/pubmed/14079489 |