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THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study
Differentiating white adipose tissue from presumptive and developing fat pads of newborn and young rats was fixed in buffered osmium tetroxide, embedded in Vestopal W, and examined in an electron microscope. Pre-adipose cells were found to be fibroblasts characterized by their spindle shape, long te...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1963
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106313/ https://www.ncbi.nlm.nih.gov/pubmed/14064115 |
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author | Napolitano, Leonard |
author_facet | Napolitano, Leonard |
author_sort | Napolitano, Leonard |
collection | PubMed |
description | Differentiating white adipose tissue from presumptive and developing fat pads of newborn and young rats was fixed in buffered osmium tetroxide, embedded in Vestopal W, and examined in an electron microscope. Pre-adipose cells were found to be fibroblasts characterized by their spindle shape, long tenuous cytoplasmic extensions, and profuse endoplasmic reticulum. The developmental stages traced from fibroblast to mature adipose cell show a gradual change in cell shape, an accumulation of cytoplasm and non-membrane-bounded lipid, a decrease in the endoplasmic reticulum, and a change in shape of mitochondria. Transitory glycogen appears at mid-differentiation. Numerous smooth-membraned vesicles occur in the cytoplasm throughout differentiation. Pinocytosis is constantly evident. Cells of the multilocular stage are shown to differ from brown fat cells, particularly with respect to cytoplasmic membrane systems and mitochondria. No transport of particulate lipid from the lumen of the capillary to, or within, the adipose cell was detected, nor could any cell organelle be demonstrated to be visibly related to lipid synthesis and/or deposition. |
format | Text |
id | pubmed-2106313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1963 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21063132008-05-01 THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study Napolitano, Leonard J Cell Biol Article Differentiating white adipose tissue from presumptive and developing fat pads of newborn and young rats was fixed in buffered osmium tetroxide, embedded in Vestopal W, and examined in an electron microscope. Pre-adipose cells were found to be fibroblasts characterized by their spindle shape, long tenuous cytoplasmic extensions, and profuse endoplasmic reticulum. The developmental stages traced from fibroblast to mature adipose cell show a gradual change in cell shape, an accumulation of cytoplasm and non-membrane-bounded lipid, a decrease in the endoplasmic reticulum, and a change in shape of mitochondria. Transitory glycogen appears at mid-differentiation. Numerous smooth-membraned vesicles occur in the cytoplasm throughout differentiation. Pinocytosis is constantly evident. Cells of the multilocular stage are shown to differ from brown fat cells, particularly with respect to cytoplasmic membrane systems and mitochondria. No transport of particulate lipid from the lumen of the capillary to, or within, the adipose cell was detected, nor could any cell organelle be demonstrated to be visibly related to lipid synthesis and/or deposition. The Rockefeller University Press 1963-09-01 /pmc/articles/PMC2106313/ /pubmed/14064115 Text en Copyright © 1963 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Napolitano, Leonard THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study |
title | THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study |
title_full | THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study |
title_fullStr | THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study |
title_full_unstemmed | THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study |
title_short | THE DIFFERENTIATION OF WHITE ADIPOSE CELLS : An Electron Microscope Study |
title_sort | differentiation of white adipose cells : an electron microscope study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106313/ https://www.ncbi.nlm.nih.gov/pubmed/14064115 |
work_keys_str_mv | AT napolitanoleonard thedifferentiationofwhiteadiposecellsanelectronmicroscopestudy AT napolitanoleonard differentiationofwhiteadiposecellsanelectronmicroscopestudy |