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INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments

The effects of proteolytic enzymes, ribonuclease, and deoxyribonuclease upon a fibrous component of chick embryo mitochondria, which was previously shown to have many fixation and staining properties characteristic of the bacterial nucleoplasm, are reported. Pepsin digestion of formaldehyde-fixed ti...

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Autores principales: Nass, Sylvan, Nass, Margit M. K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106332/
https://www.ncbi.nlm.nih.gov/pubmed/14086139
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author Nass, Sylvan
Nass, Margit M. K.
author_facet Nass, Sylvan
Nass, Margit M. K.
author_sort Nass, Sylvan
collection PubMed
description The effects of proteolytic enzymes, ribonuclease, and deoxyribonuclease upon a fibrous component of chick embryo mitochondria, which was previously shown to have many fixation and staining properties characteristic of the bacterial nucleoplasm, are reported. Pepsin digestion of formaldehyde-fixed tissues removed the membranes and matrices of mitochondria, but a pepsin-resistant fibrous material remained which was heavily stained by uranyl and lead ions. Experiments on a DNA "model system" showed that DNA treated with osmium tetroxide can be depolymerized by deoxyribonuclease. Zinc ions strongly inhibited the depolymerization of DNA. Digestion of osmium tetroxide-fixed tissues (fixed only briefly) with deoxyribonuclease for 1 hour greatly reduced the Feulgen staining of the nuclei, and after 4 hours the Feulgen reaction was completely abolished. The reduction and the disappearance of the Feulgen reaction in nuclei was paralleled by partial to complete digestion of the mitochondrial fibers in the regions studied (after 1 and 4 hours, respectively), without any other obvious changes in cellular structures. When deoxyribonuclease was inhibited by the addition of zinc ions, the nuclear Feulgen reaction was not diminished, nor were the mitochondrial fibers removed. Buffer control incubations for deoxyribonuclease and ribonuclease did not alter the structure or staining properties of the mitochondrial fibers, nor did incubation with ribonuclease. The latter reaction digested the cytoplasmic and nucleolar ribosomes after a 4-hour incubation period, in parallel with the abolishment of toluidine blue staining. The results contribute further evidence that these mitochondria contain deoxyribonucleic acid.
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spelling pubmed-21063322008-05-01 INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments Nass, Sylvan Nass, Margit M. K. J Cell Biol Article The effects of proteolytic enzymes, ribonuclease, and deoxyribonuclease upon a fibrous component of chick embryo mitochondria, which was previously shown to have many fixation and staining properties characteristic of the bacterial nucleoplasm, are reported. Pepsin digestion of formaldehyde-fixed tissues removed the membranes and matrices of mitochondria, but a pepsin-resistant fibrous material remained which was heavily stained by uranyl and lead ions. Experiments on a DNA "model system" showed that DNA treated with osmium tetroxide can be depolymerized by deoxyribonuclease. Zinc ions strongly inhibited the depolymerization of DNA. Digestion of osmium tetroxide-fixed tissues (fixed only briefly) with deoxyribonuclease for 1 hour greatly reduced the Feulgen staining of the nuclei, and after 4 hours the Feulgen reaction was completely abolished. The reduction and the disappearance of the Feulgen reaction in nuclei was paralleled by partial to complete digestion of the mitochondrial fibers in the regions studied (after 1 and 4 hours, respectively), without any other obvious changes in cellular structures. When deoxyribonuclease was inhibited by the addition of zinc ions, the nuclear Feulgen reaction was not diminished, nor were the mitochondrial fibers removed. Buffer control incubations for deoxyribonuclease and ribonuclease did not alter the structure or staining properties of the mitochondrial fibers, nor did incubation with ribonuclease. The latter reaction digested the cytoplasmic and nucleolar ribosomes after a 4-hour incubation period, in parallel with the abolishment of toluidine blue staining. The results contribute further evidence that these mitochondria contain deoxyribonucleic acid. The Rockefeller University Press 1963-12-01 /pmc/articles/PMC2106332/ /pubmed/14086139 Text en Copyright © 1963 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Nass, Sylvan
Nass, Margit M. K.
INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments
title INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments
title_full INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments
title_fullStr INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments
title_full_unstemmed INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments
title_short INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS : II. Enzymatic and Other Hydrolytic Treatments
title_sort intramitochondrial fibers with dna characteristics : ii. enzymatic and other hydrolytic treatments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106332/
https://www.ncbi.nlm.nih.gov/pubmed/14086139
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