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THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS
The sequence in which various regions of the chromosomes of human blood cells complete DNA synthesis in vitro has been studied through the use of H(3)-thymidine labeling and autoradiography. Certain of its aspects have been defined, and these may serve as a basis for comparing the pattern of synthes...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1964
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106348/ https://www.ncbi.nlm.nih.gov/pubmed/14105215 |
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author | German, James |
author_facet | German, James |
author_sort | German, James |
collection | PubMed |
description | The sequence in which various regions of the chromosomes of human blood cells complete DNA synthesis in vitro has been studied through the use of H(3)-thymidine labeling and autoradiography. Certain of its aspects have been defined, and these may serve as a basis for comparing the pattern of synthesis in cells of other tissues. In general, the long chromosomes continue replication later than the short ones. Variability of the sequence has been prominent. One pair from Group 13–15 and pair No. 17 complete replication early. In certain other chromosomes, replication is very active late in the S period, e.g. one X of the female cell, the Y of the male cell, two of Group 4–5, two of Group 13–15, the Nos. 16, and the Nos. 18. In the normal human female a striking correlation exists between the late replication of one of the X chromosomes, condensation during the intermitotic period, and presumed genetical inactivation. The pattern of replication characterizes certain chromosomes whose structural features alone are non-distinctive, and it may be useful in studies of cells in which a chromosomal aberration occurs. |
format | Text |
id | pubmed-2106348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1964 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21063482008-05-01 THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS German, James J Cell Biol Article The sequence in which various regions of the chromosomes of human blood cells complete DNA synthesis in vitro has been studied through the use of H(3)-thymidine labeling and autoradiography. Certain of its aspects have been defined, and these may serve as a basis for comparing the pattern of synthesis in cells of other tissues. In general, the long chromosomes continue replication later than the short ones. Variability of the sequence has been prominent. One pair from Group 13–15 and pair No. 17 complete replication early. In certain other chromosomes, replication is very active late in the S period, e.g. one X of the female cell, the Y of the male cell, two of Group 4–5, two of Group 13–15, the Nos. 16, and the Nos. 18. In the normal human female a striking correlation exists between the late replication of one of the X chromosomes, condensation during the intermitotic period, and presumed genetical inactivation. The pattern of replication characterizes certain chromosomes whose structural features alone are non-distinctive, and it may be useful in studies of cells in which a chromosomal aberration occurs. The Rockefeller University Press 1964-01-01 /pmc/articles/PMC2106348/ /pubmed/14105215 Text en Copyright © 1964 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article German, James THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS |
title | THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS |
title_full | THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS |
title_fullStr | THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS |
title_full_unstemmed | THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS |
title_short | THE PATTERN OF DNA SYNTHESIS IN THE CHROMOSOMES OF HUMAN BLOOD CELLS |
title_sort | pattern of dna synthesis in the chromosomes of human blood cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106348/ https://www.ncbi.nlm.nih.gov/pubmed/14105215 |
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