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THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE
Thin sections of embryonic avian bone decalcify during preparation for electron microscopy, creating a false impression of mineral distribution. The results of the experiments reported herein show that viscous embedding materials do not penetrate compact formed bone, and so, in thin sections, the ca...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1964
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106350/ https://www.ncbi.nlm.nih.gov/pubmed/14105207 |
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author | Boothroyd, B. |
author_facet | Boothroyd, B. |
author_sort | Boothroyd, B. |
collection | PubMed |
description | Thin sections of embryonic avian bone decalcify during preparation for electron microscopy, creating a false impression of mineral distribution. The results of the experiments reported herein show that viscous embedding materials do not penetrate compact formed bone, and so, in thin sections, the calcium apatite crystals may be leeched out by water, both in the collecting trough and in aqueous solutions of stains used to enhance tissue electron opacity. To prevent decalcification, a simple technique is described in which the aqueous fluids that come in contact with thin sections are saturated with respect to calcium and phosphate ions, thereby preventing solution of the bone mineral. The theoretical basis of this technique is briefly discussed. |
format | Text |
id | pubmed-2106350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1964 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21063502008-05-01 THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE Boothroyd, B. J Cell Biol Article Thin sections of embryonic avian bone decalcify during preparation for electron microscopy, creating a false impression of mineral distribution. The results of the experiments reported herein show that viscous embedding materials do not penetrate compact formed bone, and so, in thin sections, the calcium apatite crystals may be leeched out by water, both in the collecting trough and in aqueous solutions of stains used to enhance tissue electron opacity. To prevent decalcification, a simple technique is described in which the aqueous fluids that come in contact with thin sections are saturated with respect to calcium and phosphate ions, thereby preventing solution of the bone mineral. The theoretical basis of this technique is briefly discussed. The Rockefeller University Press 1964-01-01 /pmc/articles/PMC2106350/ /pubmed/14105207 Text en Copyright © 1964 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Boothroyd, B. THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE |
title | THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE |
title_full | THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE |
title_fullStr | THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE |
title_full_unstemmed | THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE |
title_short | THE PROBLEM OF DEMINERALISATION IN THIN SECTIONS OF FULLY CALCIFIED BONE |
title_sort | problem of demineralisation in thin sections of fully calcified bone |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106350/ https://www.ncbi.nlm.nih.gov/pubmed/14105207 |
work_keys_str_mv | AT boothroydb theproblemofdemineralisationinthinsectionsoffullycalcifiedbone AT boothroydb problemofdemineralisationinthinsectionsoffullycalcifiedbone |