CORRECTION OF POLYRIBOSOME DISTRIBUTIONS AS OBSERVED IN CELL SECTIONS BY ELECTRON MICROSCOPY

Clusters of ribosomes observed by electron microscopy in thin sections of rabbit reticulocytes are of the same order of size as the section thickness of 600 A. Many of the observed clusters must therefore have been transected by the section surfaces and observed as clusters containing fewer ribosome...

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Detalles Bibliográficos
Autor principal: Perl, William
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106464/
https://www.ncbi.nlm.nih.gov/pubmed/14206426
Descripción
Sumario:Clusters of ribosomes observed by electron microscopy in thin sections of rabbit reticulocytes are of the same order of size as the section thickness of 600 A. Many of the observed clusters must therefore have been transected by the section surfaces and observed as clusters containing fewer ribosomes. A probability method of correcting for this effect is given. Comparison of the results with grid observations of ribosome distributions indicates sufficiently good agreement for application to cell section observations.

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