CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements

The application of electron microscope autoradiography to Amoeba proteus cells labeled with tritiated thymidine has permitted the identification of morphologically distinct particles in the cytoplasm as the sites of incorporated DNA precursor. The particles correspond to those previously described f...

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Detalles Bibliográficos
Autores principales: Wolstenholme, D. R., Plaut, W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1964
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106471/
https://www.ncbi.nlm.nih.gov/pubmed/14208356
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author Wolstenholme, D. R.
Plaut, W.
author_facet Wolstenholme, D. R.
Plaut, W.
author_sort Wolstenholme, D. R.
collection PubMed
description The application of electron microscope autoradiography to Amoeba proteus cells labeled with tritiated thymidine has permitted the identification of morphologically distinct particles in the cytoplasm as the sites of incorporated DNA precursor. The particles correspond to those previously described from light microscope studies, with respect to both H(3)Tdr incorporation and distribution in centrifugally stratified amoebae. Ingested bacteria differ from the particles, in morphology as well as in the absence of associated label. Attempts to introduce a normal particle labeling pattern by incubating amoebae with labeled sediment derived from used amoeba medium failed. The resultant conclusion, that the particles are maintained in the amoeba by self-duplication, is supported by the presence of particles in configurations suggestive of division.
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spelling pubmed-21064712008-05-01 CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements Wolstenholme, D. R. Plaut, W. J Cell Biol Article The application of electron microscope autoradiography to Amoeba proteus cells labeled with tritiated thymidine has permitted the identification of morphologically distinct particles in the cytoplasm as the sites of incorporated DNA precursor. The particles correspond to those previously described from light microscope studies, with respect to both H(3)Tdr incorporation and distribution in centrifugally stratified amoebae. Ingested bacteria differ from the particles, in morphology as well as in the absence of associated label. Attempts to introduce a normal particle labeling pattern by incubating amoebae with labeled sediment derived from used amoeba medium failed. The resultant conclusion, that the particles are maintained in the amoeba by self-duplication, is supported by the presence of particles in configurations suggestive of division. The Rockefeller University Press 1964-09-01 /pmc/articles/PMC2106471/ /pubmed/14208356 Text en Copyright © 1964 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wolstenholme, D. R.
Plaut, W.
CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements
title CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements
title_full CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements
title_fullStr CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements
title_full_unstemmed CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements
title_short CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS : III. Further Studies on the Nature of the DNA-Containing Elements
title_sort cytoplasmic dna synthesis in amoeba proteus : iii. further studies on the nature of the dna-containing elements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106471/
https://www.ncbi.nlm.nih.gov/pubmed/14208356
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