Cargando…
THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and con...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1965
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106695/ https://www.ncbi.nlm.nih.gov/pubmed/4285675 |
_version_ | 1782138569773547520 |
---|---|
author | Granick, S. Levere, Richard D. |
author_facet | Granick, S. Levere, Richard D. |
author_sort | Granick, S. |
collection | PubMed |
description | A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and converts the heme to a porphyrin which fluoresces and is readily visualized. With this technique, hemoglobin has been demonstrated in the nuclei of avian erythrocytes, and in the nuclei of human normoblasts at an earlier stage than previously described. In addition, hemoproteins, presumably cytochromes, have been detected in the cytoplasm and nuclei of myelocytes, in thymus lymphocyte nuclei, in chick embryo liver cytoplasm, and in chick embryo somites. |
format | Text |
id | pubmed-2106695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1965 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21066952008-05-01 THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE Granick, S. Levere, Richard D. J Cell Biol Article A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and converts the heme to a porphyrin which fluoresces and is readily visualized. With this technique, hemoglobin has been demonstrated in the nuclei of avian erythrocytes, and in the nuclei of human normoblasts at an earlier stage than previously described. In addition, hemoproteins, presumably cytochromes, have been detected in the cytoplasm and nuclei of myelocytes, in thymus lymphocyte nuclei, in chick embryo liver cytoplasm, and in chick embryo somites. The Rockefeller University Press 1965-07-01 /pmc/articles/PMC2106695/ /pubmed/4285675 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Granick, S. Levere, Richard D. THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE |
title | THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE |
title_full | THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE |
title_fullStr | THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE |
title_full_unstemmed | THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE |
title_short | THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE |
title_sort | intracellular localization of heme by a fluorescence technique |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106695/ https://www.ncbi.nlm.nih.gov/pubmed/4285675 |
work_keys_str_mv | AT granicks theintracellularlocalizationofhemebyafluorescencetechnique AT levererichardd theintracellularlocalizationofhemebyafluorescencetechnique AT granicks intracellularlocalizationofhemebyafluorescencetechnique AT levererichardd intracellularlocalizationofhemebyafluorescencetechnique |