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THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE

A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and con...

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Detalles Bibliográficos
Autores principales: Granick, S., Levere, Richard D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106695/
https://www.ncbi.nlm.nih.gov/pubmed/4285675
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author Granick, S.
Levere, Richard D.
author_facet Granick, S.
Levere, Richard D.
author_sort Granick, S.
collection PubMed
description A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and converts the heme to a porphyrin which fluoresces and is readily visualized. With this technique, hemoglobin has been demonstrated in the nuclei of avian erythrocytes, and in the nuclei of human normoblasts at an earlier stage than previously described. In addition, hemoproteins, presumably cytochromes, have been detected in the cytoplasm and nuclei of myelocytes, in thymus lymphocyte nuclei, in chick embryo liver cytoplasm, and in chick embryo somites.
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spelling pubmed-21066952008-05-01 THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE Granick, S. Levere, Richard D. J Cell Biol Article A new technique for the intracellular localization of minute amounts of heme and hemoproteins is described. The specimen is treated with 1.5 M perchloric acid in the presence of SH groups, followed by ultraviolet light irradiation in a fluorescence microscope. This fixes the proteins in situ and converts the heme to a porphyrin which fluoresces and is readily visualized. With this technique, hemoglobin has been demonstrated in the nuclei of avian erythrocytes, and in the nuclei of human normoblasts at an earlier stage than previously described. In addition, hemoproteins, presumably cytochromes, have been detected in the cytoplasm and nuclei of myelocytes, in thymus lymphocyte nuclei, in chick embryo liver cytoplasm, and in chick embryo somites. The Rockefeller University Press 1965-07-01 /pmc/articles/PMC2106695/ /pubmed/4285675 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Granick, S.
Levere, Richard D.
THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
title THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
title_full THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
title_fullStr THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
title_full_unstemmed THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
title_short THE INTRACELLULAR LOCALIZATION OF HEME BY A FLUORESCENCE TECHNIQUE
title_sort intracellular localization of heme by a fluorescence technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106695/
https://www.ncbi.nlm.nih.gov/pubmed/4285675
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