COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase

Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate...

Descripción completa

Detalles Bibliográficos
Autores principales: Baudhuin, Pierre, Beaufay, Henri, de Duve, Christian
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106697/
https://www.ncbi.nlm.nih.gov/pubmed/4379260
Descripción
Sumario:Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.

Ejemplares similares