COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase

Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate...

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Detalles Bibliográficos
Autores principales: Baudhuin, Pierre, Beaufay, Henri, de Duve, Christian
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106697/
https://www.ncbi.nlm.nih.gov/pubmed/4379260
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author Baudhuin, Pierre
Beaufay, Henri
de Duve, Christian
author_facet Baudhuin, Pierre
Beaufay, Henri
de Duve, Christian
author_sort Baudhuin, Pierre
collection PubMed
description Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.
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spelling pubmed-21066972008-05-01 COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase Baudhuin, Pierre Beaufay, Henri de Duve, Christian J Cell Biol Article Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane. The Rockefeller University Press 1965-07-01 /pmc/articles/PMC2106697/ /pubmed/4379260 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Baudhuin, Pierre
Beaufay, Henri
de Duve, Christian
COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase
title COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase
title_full COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase
title_fullStr COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase
title_full_unstemmed COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase
title_short COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER : Analysis of Preparations Enriched in Lysosomes or in Particles Containing Urate Oxidase, D-Amino Acid Oxidase, and Catalase
title_sort combined biochemical and morphological study of particulate fractions from rat liver : analysis of preparations enriched in lysosomes or in particles containing urate oxidase, d-amino acid oxidase, and catalase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106697/
https://www.ncbi.nlm.nih.gov/pubmed/4379260
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