STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES

Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contain...

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Detalles Bibliográficos
Autores principales: Trosko, James E., Wolff, Sheldon
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106708/
https://www.ncbi.nlm.nih.gov/pubmed/5323605
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author Trosko, James E.
Wolff, Sheldon
author_facet Trosko, James E.
Wolff, Sheldon
author_sort Trosko, James E.
collection PubMed
description Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein.
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spelling pubmed-21067082008-05-01 STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES Trosko, James E. Wolff, Sheldon J Cell Biol Article Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein. The Rockefeller University Press 1965-07-01 /pmc/articles/PMC2106708/ /pubmed/5323605 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Trosko, James E.
Wolff, Sheldon
STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
title STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
title_full STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
title_fullStr STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
title_full_unstemmed STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
title_short STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES
title_sort strandedness of vicia faba chromosomes as revealed by enzyme digestion studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106708/
https://www.ncbi.nlm.nih.gov/pubmed/5323605
work_keys_str_mv AT troskojamese strandednessofviciafabachromosomesasrevealedbyenzymedigestionstudies
AT wolffsheldon strandednessofviciafabachromosomesasrevealedbyenzymedigestionstudies

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