THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM

The effect of penicillin on the structure of Bacillus megaterium cells was followed in media with and without osmotic stabilization. In peptone without osmotic support the cells showed a distortion of the normal membrane-wall relationship by 20 minutes. This appeared to be a combination of both memb...

Descripción completa

Detalles Bibliográficos
Autores principales: Fitz-James, Philip, Hancock, Ronald
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106740/
https://www.ncbi.nlm.nih.gov/pubmed/4956030
_version_ 1782138585516867584
author Fitz-James, Philip
Hancock, Ronald
author_facet Fitz-James, Philip
Hancock, Ronald
author_sort Fitz-James, Philip
collection PubMed
description The effect of penicillin on the structure of Bacillus megaterium cells was followed in media with and without osmotic stabilization. In peptone without osmotic support the cells showed a distortion of the normal membrane-wall relationship by 20 minutes. This appeared to be a combination of both membrane distortion and cytoplasmic leakage. Lytic changes quickly followed. With osmotic support a clean-cut lesion at the transverse-septal site developed by 10 minutes' growth in penicillin. The membrane lost its normal relationship to the cell wall and formed a pocket which was filled with a fibrous material which appeared to be unorganized wall mucopeptide. The pocket of fibers enlarged until the cell either lysed or formed a protoplast.
format Text
id pubmed-2106740
institution National Center for Biotechnology Information
language English
publishDate 1965
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21067402008-05-01 THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM Fitz-James, Philip Hancock, Ronald J Cell Biol Article The effect of penicillin on the structure of Bacillus megaterium cells was followed in media with and without osmotic stabilization. In peptone without osmotic support the cells showed a distortion of the normal membrane-wall relationship by 20 minutes. This appeared to be a combination of both membrane distortion and cytoplasmic leakage. Lytic changes quickly followed. With osmotic support a clean-cut lesion at the transverse-septal site developed by 10 minutes' growth in penicillin. The membrane lost its normal relationship to the cell wall and formed a pocket which was filled with a fibrous material which appeared to be unorganized wall mucopeptide. The pocket of fibers enlarged until the cell either lysed or formed a protoplast. The Rockefeller University Press 1965-08-01 /pmc/articles/PMC2106740/ /pubmed/4956030 Text en Copyright © 1965 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Fitz-James, Philip
Hancock, Ronald
THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM
title THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM
title_full THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM
title_fullStr THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM
title_full_unstemmed THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM
title_short THE INITIAL STRUCTURAL LESION OF PENICILLIN ACTION IN BACILLUS MEGATERIUM
title_sort initial structural lesion of penicillin action in bacillus megaterium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106740/
https://www.ncbi.nlm.nih.gov/pubmed/4956030
work_keys_str_mv AT fitzjamesphilip theinitialstructurallesionofpenicillinactioninbacillusmegaterium
AT hancockronald theinitialstructurallesionofpenicillinactioninbacillusmegaterium
AT fitzjamesphilip initialstructurallesionofpenicillinactioninbacillusmegaterium
AT hancockronald initialstructurallesionofpenicillinactioninbacillusmegaterium

Ejemplares similares