THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS

Bovine retinal outer segment fragments were isolated by density gradient centrifugation in a high centrifugal field. Assays of the final preparation for enzymes of the mitochondrial respiratory chain indicated mitochondrial contamination not in excess of 1 per cent. Glucose-6-phosphatase and TPNH-cy...

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Detalles Bibliográficos
Autor principal: McConnell, David G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106757/
https://www.ncbi.nlm.nih.gov/pubmed/4287275
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author McConnell, David G.
author_facet McConnell, David G.
author_sort McConnell, David G.
collection PubMed
description Bovine retinal outer segment fragments were isolated by density gradient centrifugation in a high centrifugal field. Assays of the final preparation for enzymes of the mitochondrial respiratory chain indicated mitochondrial contamination not in excess of 1 per cent. Glucose-6-phosphatase and TPNH-cytochrome c reductase activities, presumably diagnostic for microsomes, were also absent. Electron micrographs did not disclose the presence of significant numbers of particles other than fragments of the outer segment discs. The red fragments were characterized by an ascorbate-oxidizing system and a high lipid content.
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spelling pubmed-21067572008-05-01 THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS McConnell, David G. J Cell Biol Article Bovine retinal outer segment fragments were isolated by density gradient centrifugation in a high centrifugal field. Assays of the final preparation for enzymes of the mitochondrial respiratory chain indicated mitochondrial contamination not in excess of 1 per cent. Glucose-6-phosphatase and TPNH-cytochrome c reductase activities, presumably diagnostic for microsomes, were also absent. Electron micrographs did not disclose the presence of significant numbers of particles other than fragments of the outer segment discs. The red fragments were characterized by an ascorbate-oxidizing system and a high lipid content. The Rockefeller University Press 1965-12-01 /pmc/articles/PMC2106757/ /pubmed/4287275 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
McConnell, David G.
THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
title THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
title_full THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
title_fullStr THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
title_full_unstemmed THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
title_short THE ISOLATION OF RETINAL OUTER SEGMENT FRAGMENTS
title_sort isolation of retinal outer segment fragments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106757/
https://www.ncbi.nlm.nih.gov/pubmed/4287275
work_keys_str_mv AT mcconnelldavidg theisolationofretinaloutersegmentfragments
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