NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS

Young wheat roots were labeled with (32)P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepare...

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Detalles Bibliográficos
Autores principales: Hotta, Yasuo, Bassel, Alix, Stern, Herbert
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106767/
https://www.ncbi.nlm.nih.gov/pubmed/5885425
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author Hotta, Yasuo
Bassel, Alix
Stern, Herbert
author_facet Hotta, Yasuo
Bassel, Alix
Stern, Herbert
author_sort Hotta, Yasuo
collection PubMed
description Young wheat roots were labeled with (32)P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells.
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spelling pubmed-21067672008-05-01 NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS Hotta, Yasuo Bassel, Alix Stern, Herbert J Cell Biol Article Young wheat roots were labeled with (32)P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells. The Rockefeller University Press 1965-12-01 /pmc/articles/PMC2106767/ /pubmed/5885425 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hotta, Yasuo
Bassel, Alix
Stern, Herbert
NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
title NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
title_full NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
title_fullStr NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
title_full_unstemmed NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
title_short NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS
title_sort nuclear dna and cytoplasmic dna from tissues of higher plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106767/
https://www.ncbi.nlm.nih.gov/pubmed/5885425
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AT basselalix nucleardnaandcytoplasmicdnafromtissuesofhigherplants
AT sternherbert nucleardnaandcytoplasmicdnafromtissuesofhigherplants

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